rBet v 2 (Betula verrucosa 2.0101)

rBet v 2 (Betula verrucosa 2.0101)
Regulatory status
RUO
Format
Purified Azide Free
Application
ELISA, FC
Variant
0.1 mg
10-P177-C100
Ask for delivery term HERE
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Description
Images
Application
ELISA, FC
Description
Recombinant protein rBet v 2 is expressed in Escherichia coli. DNA sequence encoding 152 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 16,2 kDa.
Contents
Recombinant protein rBet v 2 - 1 vial (0.1 mg), lyophilized.
Reconstitute with sterile deionized water with or without azide.
Preparation
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Formulation
Lyophilized from a 0.22 μm filtered solution in Tris buffer (100 mM Tris, 150 mM NaCl, pH 8), preservative- and carrier-free. Purity > 95% (HPLC method).
Storage and handling
The product is shipped at ambient temperature. Transport conditions, including short-term elevated temperature, do not impair product performance characteristics or do not affect stated product expiry date. Upon receipt, store and handle as directed.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
Licence note
Manufactured by Apronex Ltd., an EXBIO company.
UniProt ID P25816
10-P177_ELISA
ELISA (enzyme-linked immunosorbent assay) test was designed to prove the bond between the coated target recombinant allergen rBet v 2 and allergen-specific human plasma IgG4 antibodies of Betula verrucosa positive donor. A measurable signal was subsequently generated by the addition of biotin labeled anti-human IgG4 (detection) antibody, Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated rBet v 2.
10-P177_čistota
Recombinant allergen rBet v 2 purity verification. 5 µg of rBet v 2 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
10-P177_FC_Dot-plot
Flow cytometry dot-plot staining pattern of rBet v 2 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (MEM-259) FITC and anti-human CD203c (NP4D6) PE antibodies of BasoFlowEx kit.
Variant
0.1 mg
10-P177-C100
Ask for delivery term HERE
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

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