Our Response to COVID-19

Although our company is not able to provide SARS-CoV-2 screening assays (e.g. molecular RT-qPCR assays or serological tests to identify IgM and IgG antibodies to the coronavirus), we are proud to partner with group of Czech medical faculty hospitals to support healthcare professionals efforts in better understanding the COVID-19 patient immune system status, which can possibly aid to the management of medical intervention.

We developed products for multicolor flow-cytometric immunophenotyping of cells of the human immune system from peripheral blood. Design of the new products reflects the recently published report on the kinetics of immune responses in relation to clinical and virological features of a COVID-19 patient that required hospitalization (1) and other studies observing immunologic features or pathological findings in severe COVID-19 patients (2, 3).

Monoclonal antibody conjugate multicolor panels are designed to enable characterization of antibody secreting cells (using DryFlowEx ASC Screening Kit) and subpopulations of activated T cells, including circulating follicular helper cells (using DryFlowEx ACT T Screening Kit) in EDTA anticoagulated peripheral blood.

In order to minimize hands-on-time and contacts with the infectious blood specimen, the reagents are provided in a ready-to-use single-test tube format. Each tube (standard 12 x 75 mm flow cytometry tube) contains a layer with the dried pre-formulated mixture of antibody conjugates for direct labeling of target cells.
 

Simple laboratory workflow enables operator to follow just three easy steps:

  • add whole blood specimen to the test tube, vortex and incubate;

  • lyse erythrocytes with EXCELLYSE Easy reagent and wash;

  • acquire sample and get cytometry data
     

DryFlowEx ASC Screen Kit (ED7704)

CD45 Pacific Blue™ / IgD FITC / CD27 PE / CD24 PerCP-Cy™5.5 / CD19 PE-Cy™7 / CD21 APC / CD38 APC-Cy™7

50x single-test tube (packed in 10 moisture-protective aluminum pouches, 5 tubes each)
1x set of compensation tubes (packed in 1 moisture-protective aluminum pouch, 7 tubes; Identical fluorochrome-labeled antibodies in the set enable easy and accurate compensation procedure)
 

DryFlowEx ACT T Screen Kit (ED7705)

CD45 Pacific Blue™ / CD8 Pacific Orange™ / CD4 FITC / PD-1 PE / HLA-DR PerCP-Cy™5.5 / CD3 PE-Cy™7 / CXCR5 APC / CD38 APC-Cy™7

50x single-test tube (packed in 10 moisture-protective aluminum pouches, 5 tubes each)
1x set of compensation tubes (packed in 1 moisture-protective aluminum pouch, 8 tubes; Identical fluorochrome-labeled antibodies in the set enable easy and accurate compensation procedure)

 

FOR SPECIAL  25% DISCOUNT ON PRODUCTS TO SUPPORT LABORATORIES TESTING COVID-19 PATIENT SAMPLES PLEASE MENTION ON YOUR PURCHASE ORDER CODE CQ1200287.
 

Technical and application support: technical@exbio.cz
Availability of products, price quotations: info@exbio.cz
Orders: orders@exbio.cz

 

(1) Thevarajan, I., Nguyen, T.H.O., Koutsakos, M. et al. Breadth of concomitant immune responses prior to patient recovery: a case report of non-severe COVID-19. Nat Med (2020).
(2) Xu Z, Shi L, Wang Y, et al. Pathological findings of COVID-19 associated with acute respiratory distress syndrome. Lancet Respir Med (2020).
(3) Guang Chen G, Zhao J, Ning Q, et al. Clinical and immunologic features in severe and moderate Coronavirus Disease 2019. J Clin Invest. (2020).