DryFlowEx ASC Screen Kit

DryFlowEx ASC Screen Kit
Regulatory status
RUO
Reactivity
Human
Application
FC (QC tested)
Variant
50 tests
ED7704
In stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD
Product details
Images
Application
FC (QC tested)
Reactivity
Human
Description
DryFlowEx ASC Screen Kit is designed for flow cytometry screening of antibody secreting B cells (ASC) during virus infection.

Multicolor panel of antibody conjugates CD45/ IgD/ CD27/ CD24/ CD19/ CD21/ CD38 dried in a single flow cytometry tube enables immunophenotyping of differentiated B lymphocyte subsets in EDTA-anticoagulated human blood.
Contents
50 single-test tubes (ED7704-1T ASC Screen Tube); each 5 pcs of single-test tubes are packed with dessicant in moisture-protective aluminum pouches (ED7704-5T ASC Screen 5T Pack)

1 set of compensation tubes (ED7704-CT ASC Screen Compensation Tubes Pack)
ED7704-CT1 CD45 Pacific Blue™
ED7704-CT2 IgD FITC
ED7705-CT3 CD27 PE
ED7704-CT4 CD24 PerCP-Cy5.5
ED7704-CT5 CD19 PE-Cy7
ED7704-CT6 CD21 APC
ED7704-CT7 CD38 APC-Cy7
Identical fluorochrome-labeled antibodies allow easy and accurate compensation procedure.
Storage and handling
Store the product at 20-30°C. Expiration is stated on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchsing a license to this product for purposes orther than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
ED7704_COVID-19-patient
Example of the kinetics of frequency of immune cells in a COVID-19 patient that required hospitalization. Upper panel shows the gating procedure for identification of antibody secreting cells (ASC) subpopulations in peripheral whole blood of a patient. Lower panel shows cell frequency (% of B cells) measured in several time points during the hospitalization of a patient.
ED7704_Gate-ovací strategie
Flow cytometry phenotyping strategy for identification of Antibody Secreting Cells (ASC) in normal human peripheral whole blood. 1. Singlet cell events 2. Leukocytes 3. Lymphocytes 4. CD19+ B lymphocytes 5. CD19+ CD27 Memory B lymphocytes 6. CD19+ CD27+ CD38+ Antibody Secreting Cells 7. IgD+ non-switched and IgD- class-switched Plasmablasts
ED7704_FC_Profil Značení
Flow cytometry surface staining pattern of circulating Antibody Scereting Cells (CD19+CD27+CD38+ Plasmablasts) stained using ED7704 DryFlowEx ASC B Screen kit in normal human peripheral whole blood. For each target population the frequency(% of B cells) is shown.
Variant
50 tests
ED7704
In stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD

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