rDau c 1 (Daucus carota 1.0101 )

rDau c 1 (Daucus carota 1.0101 )
Regulatory status
RUO
Format
Purified Azide Free
Application
Variant
0.1 mg
10-P193-C100
In stock
242.00 USD
Variant
0.1 mg
10-P193-C100
In stock
242.00 USD
Product details
Description
Images
SDS download
Application
Description
Recombinant protein rDau c 1 is expressed in Escherichia coli. DNA sequence encoding 167 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 17,5 kDa.
Contents
Recombinant protein rDau c 1 - 1 vial (0.1 mg), lyophilized.
Reconstitute with sterile deionized water with or without azide.
Preparation
Purified by sequential steps of ion exchange and affinity chromatography. Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0).
Storage and handling
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
UniProt ID O04298
10-P193_ELISA
ELISA (enzyme-linked immunosorbent assay) test was designed to prove the bond between the coated target recombinant allergen rDau c 1 and allergen-specific human plasma IgG4 antibodies of Daucus carota positive donor. A measurable signal was subsequently generated by the addition of biotin labeled anti-human IgG4 (detection) antibody, Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated rDau c 1.
10-P193_čistota
Recombinant allergen rDau c 1 purity verification. 5 µg of rDau c 1 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
10-P193_FC_Profil Značení
Flow cytometry dot-plot staining pattern of rDau c 1 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (MEM-259) FITC and anti-human CD203c (NP4D6) PE antibodies of BasoFlowEx kit.
Variant
0.1 mg
10-P193-C100
In stock
242.00 USD
Variant
0.1 mg
10-P193-C100
In stock
242.00 USD