rApi g 1 (Apium graveolens 1.0101)

rApi g 1 (Apium graveolens 1.0101)
Regulatory status
RUO
Format
Purified Azide Free
Application
ELISA, FC
Variant
0.1 mg
10-P149-C100
Out of stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD
Product details
Images
Application
ELISA, FC
Description
Recombinant protein r Api g 1 is expressed in Escherichia coli. DNA sequence encoding 167 AAs was fused with His-tag at the C-terminus. A calculated molecular mass of recombinant protein is 17,9 kDa.
Contents
Recombinant protein r Api g 1 - 1 vial (0.1 mg), lyophilized.
Reconstitute with sterile deionized water with or without azide.
Preparation
The protein was purified by ionex and affinity chromatography, using His-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification.
Formulation
Lyophilized from a 0.22 μm filtered solution in Hepes buffer (20 mM Hepes, 300 mM NaCl, 2% glycerol, 0.01% Tween 20, pH 7.4), preservative- and carrier-free. Purity > 95% (HPLC method).
Storage and handling
Store the lyophilized product in freezer (-20°C to -80°C). Shipping and short term storage (max. 1 month) is possible at room temperature. The expiration date of unopened product vial is indicated on the vial label.
After reconstitution prepare suitable aliquots. Reconstituted product is stable for at least 6 months when stored in freezer (-20°C to -80°C). Avoid freeze/thaw cycles.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
Licence note
Manufactured by Apronex Ltd., an EXBIO company.
10-P149_FC_Profil Značení
Flow cytometry dot-plot staining pattern of rApi g 1 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (MEM-259) FITC and anti-human CD203c (NP4D6) PE antibodies of BasoFlowEx kit.
10-P149_čistota
Recombinant allergen rApi g 1 purity verification. 5 µg of rApi g 1 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
Variant
0.1 mg
10-P149-C100
Out of stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD