Exbio — Research products — Antibodies — Microbiology and virology — Anti-ESAT-6 (M. tuberculosis) Purified

Anti-ESAT-6 (M. tuberculosis) Purified

Regulatory status

RUO

Antigen

ESAT-6 (M. tuberculosis)

Clone

PAb (491)

Format

Purified

Reactivity

Mycobacterium tuberculosis

Application

Variant

0.1 mg

11-491-C100

In stock

198.00 USD

0.025 mg

11-491-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-491-C100

In stock

198.00 USD

0.025 mg

11-491-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

References

SDS download

Isotype

Rabbit polyclonal

Specificity

The rabbit polyclonal antibody to EsaT-6 recognizes the EsaT-6 (Rv3875) protein of Mycobacterium tuberculosis and Mycobacterium bovis.

Application

Application details

Western blotting: Recommended dilution 0.5-1 μg/ml, reducing conditions.

Reactivity

Mycobacterium tuberculosis

Immunogen

EsaT-6 (Rv3875) of Mycobacterium tuberculosis

Concentration

1 mg/ml

Preparation

Purified by ligand affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ESXA, ESAT-6, Rv3875

Antigen description

The Mycobacterium tuberculosis antigen EsaT-6 (6 kDa early secretory antigen target EsxA ) causes induction of neutrophil necrosis. This protein acts as a leukocidin, which may facilitate bacterial avoidance of the antimicrobial action of the immune system.

Entrez Gene ID 886209

UniProt ID P9WNK7

General references:

Francis RJ, Butler RE, Stewart GR: Mycobacterium tuberculosis ESAT-6 is a leukocidin causing Ca2+ influx, necrosis and neutrophil extracellular trap formation. Cell Death Dis. 2014 Oct 16;5:e1474.
PubMed

Arlehamn CS, Sidney J, Henderson R, Greenbaum JA, James EA, Moutaftsi M, Coler R, McKinney DM, Park D, Taplitz R, Kwok WW, Grey H, Peters B, Sette A: Dissecting mechanisms of immunodominance to the common tuberculosis antigens ESAT-6, CFP10, Rv2031c (hspX), Rv2654c (TB7.7), and Rv1038c (EsxJ). J Immunol. 2012 May 15;188(10):5020-31.
PubMed