Anti-Perforin Purified

Anti-Perforin Purified
Regulatory status
RUO
Antigen
Perforin
Clone
dG9
Format
Purified
Reactivity
Human, Cow
Variant
0.1 mg
11-765-C100
In stock
165.00 USD

0.025 mg
11-765-C025
Delivery 1 week
82.50 USD
Variant
0.1 mg
11-765-C100
In stock
165.00 USD

0.025 mg
11-765-C025
Delivery 1 week
82.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2b kappa
Specificity
The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
Application details
Flow cytometry: Recommended dilution: 3-12 μg/ml. Intracellular staining.
Reactivity
Human, Cow
Negative species
Mouse
Immunogen
purified granules from human YT lymphoma cell line
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
PRF1, P1, PFP, HPLH2
Antigen description
Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.
Entrez Gene ID 5551
UniProt ID P14222
11-765_FC_Profil Značení
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Perforin (dG9) purified antibody (concentration in sample 2 μg/ml, GAM FITC).
11-765_FC_Histogram
Separation of perforin positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracelluar staining) of human peripheral whole blood using anti-human perforin (dG9) purified antibody (concentration in sample 2 μg/ml, GAM FITC).

Product specific references:

Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M: A CD8α(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities. Immunology. 2011 Nov;134(3):326-40.
PubMed
Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. J Mol Cell Biol. 2010 Jun;2(3):139-51.
PubMed
Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW: Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions. PLoS Pathog. 2012;8(11):e1002984.
PubMed
Hersperger AR, Makedonas G, Betts MR: Flow cytometric detection of perforin upregulation in human CD8 T cells. Cytometry A. 2008 Nov;73(11):1050-7.
PubMed
Hameed A, Olsen KJ, Cheng L, Fox WM, Hruban RH, Podack ER: Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992 May;140(5):1025-30.
PubMed
Variant
0.1 mg
11-765-C100
In stock
165.00 USD

0.025 mg
11-765-C025
Delivery 1 week
82.50 USD
Variant
0.1 mg
11-765-C100
In stock
165.00 USD

0.025 mg
11-765-C025
Delivery 1 week
82.50 USD