Exbio — Research products — Antibodies — Serum, plasma, and secreted proteins — Anti-Perforin Purified

Anti-Perforin Purified

Regulatory status

RUO

Antigen

Perforin

Clone

dG9

Format

Purified

Reactivity

Human, Cow

Application

IP, FC (QC tested), IHC-F, IHC-P, ICC

Variant

0.1 mg

11-765-C100

In stock

165.00 USD

0.025 mg

11-765-C025

Delivery 1 week

82.50 USD

Variant

0.1 mg

11-765-C100

In stock

165.00 USD

0.025 mg

11-765-C025

Delivery 1 week

82.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.

Application

IP, FC (QC tested), IHC-F, IHC-P, ICC

Application details

Flow cytometry: Recommended dilution: 3-12 μg/ml. Intracellular staining.

Reactivity

Human, Cow

Negative species

Mouse

Immunogen

purified granules from human YT lymphoma cell line

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

PRF1, P1, PFP, HPLH2

Antigen description

Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.

Entrez Gene ID 5551

UniProt ID P14222

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Perforin (dG9) purified antibody (concentration in sample 2 μg/ml, GAM FITC).

Separation of perforin positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracelluar staining) of human peripheral whole blood using anti-human perforin (dG9) purified antibody (concentration in sample 2 μg/ml, GAM FITC).

Product specific references:

Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M: A CD8α(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities. Immunology. 2011 Nov;134(3):326-40.
PubMed

Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. J Mol Cell Biol. 2010 Jun;2(3):139-51.
PubMed

Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW: Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions. PLoS Pathog. 2012;8(11):e1002984.
PubMed

Hersperger AR, Makedonas G, Betts MR: Flow cytometric detection of perforin upregulation in human CD8 T cells. Cytometry A. 2008 Nov;73(11):1050-7.
PubMed

Hameed A, Olsen KJ, Cheng L, Fox WM, Hruban RH, Podack ER: Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992 May;140(5):1025-30.
PubMed