Clinical products
Premium antibodies (GMP)
Intracellular staining
Surface staining
Hematology
Kits
Lysing solutions
Premixed antibody combinations
Single color antibodies
Immunodeficiency
Kits
Lysing solutions
Mitogens
Premixed antibody combinations
Single color antibodies
Reproductive immunology
Kits
Lysing solutions
Premixed antibody combinations
New products
Allergy
Kits
Recombinant allergens
Allergen extracts
Other allergens
Research products
Antibodies
CD and related antigens
Blood group antigens
Premixed antibody combinations
Serum, plasma, and secreted proteins
Cytoskeleton and ECM
Isotype controls
MHC antigens
Nucleus
Other intracellular antigens
Small organic molecules
Tags and labels
Microbiology and virology
Accessory reagents
Annexin V
DNA-binding probes
Lineage cocktail
Lysing solutions
Recombinant proteins
Recombinant allergens
Animal
Food
House Dust Mite
Mold
Pollen
Venom
Kits
New products
Dry Reagents
About exbio
Company
OEM and Commercial Supplies
Events and Conferences
Blog
Promotions
Inquiries
Technical resources
International distributors
Contact
Jobs
E-mail
*
Password
*
Remember me
Forgotten password
Log in
Register
E-mail
Send request
Log in
Exbio
—
Research products
—
Antibodies
—
Serum, plasma, and secreted proteins
—
Anti-Clusterin Purified
Anti-Clusterin Purified
Regulatory status
RUO
Antigen
Clusterin
Clone
Hs-3
Format
Purified
Reactivity
Human
Application
ELISA, WB, IHC(P), ICC
Variant
0.1 mg
11-421-C100
In stock
160.00 USD
0.025 mg
11-421-C025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-421-C100
In stock
160.00 USD
0.025 mg
11-421-C025
Delivery 1 week
80.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody Hs-3 recognizes human clusterin (apolipoprotein J), a conserved secreted glycoprotein.
Application
ELISA, WB, IHC(P), ICC
Application details
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: Sertoli cells.
Western blotting: Recommended dilution: 1 μg/ml; positive material: seminal plasma.
Reactivity
Human
Negative species
Dog, Cat, Pig, Cow
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Complement-associated protein SP-40, Complement cytolysis inhibitor, CLI, CLU, NA1/NA2, Apolipoprotein J, Apo-J, TRPM-2, KBP1, KUB1, CLI, AAG4, APOJ, SGP2
Antigen description
Clusterin (APO J, SGP-2, TRPM-2, SP-40, pADHC-9, CLJ, T64, GP III, XIP8) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. It is a conserved secreted glycoprotein expressed by a wide range of tissues and being implicated in many physiological processes, including e.g. lipid transportation, complement inhibition, tissue remodeling, membrane recycling, or clearence of cellular debris. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. Clusterin is able to bind and form complexes with numerous partners (immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin etc.) and is expressed in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. A genuine function of clusterin is still enigmatic.
Entrez Gene ID
1191
UniProt ID
P10909
Western blotting analysis of human clusterin using mouse monoclonal antibody Hs-3 on lysates of MCF-7 cell line and MOLT-4 cell line (clusterin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-clusterin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Clusterin was detected around 40 kDa.
General references:
Shannan B, Seifert M, Boothman DA, Tilgen W, Reichrath J: Clusterin and DNA repair: a new function in cancer for a key player in apoptosis and cell cycle control. J Mol Histol. 2006 Sep;37(5-7):183-8.
PubMed
Rodríguez-Piñeiro AM, de la Cadena MP, López-Saco A, Rodríguez-Berrocal FJ: Differential expression of serum clusterin isoforms in colorectal cancer. Mol Cell Proteomics. 2006 Sep;5(9):1647-57.
PubMed
Pajak B, Orzechowski A: Clusterin: the missing link in the calcium-dependent resistance of cancer cells to apoptogenic stimuli. Postepy Hig Med Dosw (Online). 2006;60:45-51.
PubMed
Cochrane DR, Wang Z, Muramaki M, Gleave ME, Nelson CC: Differential regulation of clusterin and its isoforms by androgens in prostate cells. J Biol Chem. 2007 Jan 26;282(4):2278-87.
PubMed
Schepeler T, Mansilla F, Christensen LL, Orntoft TF, Andersen CL: Clusterin expression can be modulated by changes in TCF1-mediated Wnt signaling. J Mol Signal. 2007 Jul 16;2:6.
PubMed
Product specific references:
Capkova J, Geussova G, Peknicova J: New monoclonal antibody to human apolipoprotein J. Folia Biol (Praha). 2002;48(1):40-2.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15(11):909-912.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
11-421-C100
In stock
160.00 USD
0.025 mg
11-421-C025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-421-C100
In stock
160.00 USD
0.025 mg
11-421-C025
Delivery 1 week
80.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
Continue to checkout
×
Zavřít