Anti-Clusterin Purified

Anti-Clusterin Purified
Regulatory status
RUO
Antigen
Clusterin
Clone
Hs-3
Format
Purified
Reactivity
Human
Application
ELISA, WB, IHC(P), ICC
Variant
0.1 mg
11-421-C100
In stock

0.025 mg
11-421-C025
In stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody Hs-3 recognizes human clusterin (apolipoprotein J).
Application
ELISA, WB, IHC(P), ICC
Reactivity
Human
Negative species
Dog, Cat, Pig, Cow
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Other names
Complement-associated protein SP-40, Complement cytolysis inhibitor, CLI, CLU, NA1/NA2, Apolipoprotein J, Apo-J, TRPM-2, KBP1, KUB1, CLI, AAG4, APOJ, SGP2
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-421 WB
Western blotting analysis (non-reducing conditions) of clusterin in human seminal plasma using anti-clusterin (Hs-3) purified.

General references:

Shannan B, Seifert M, Boothman DA, Tilgen W, Reichrath J: Clusterin and DNA repair: a new function in cancer for a key player in apoptosis and cell cycle control. J Mol Histol. 2006 Sep;37(5-7):183-8.
PubMed
Rodríguez-Piñeiro AM, de la Cadena MP, López-Saco A, Rodríguez-Berrocal FJ: Differential expression of serum clusterin isoforms in colorectal cancer. Mol Cell Proteomics. 2006 Sep;5(9):1647-57.
PubMed
Pajak B, Orzechowski A: Clusterin: the missing link in the calcium-dependent resistance of cancer cells to apoptogenic stimuli. Postepy Hig Med Dosw (Online). 2006;60:45-51.
PubMed
Cochrane DR, Wang Z, Muramaki M, Gleave ME, Nelson CC: Differential regulation of clusterin and its isoforms by androgens in prostate cells. J Biol Chem. 2007 Jan 26;282(4):2278-87.
PubMed
Schepeler T, Mansilla F, Christensen LL, Orntoft TF, Andersen CL: Clusterin expression can be modulated by changes in TCF1-mediated Wnt signaling. J Mol Signal. 2007 Jul 16;2:6.
PubMed

Product specific references:

Capkova J, Geussova G, Peknicova J: New monoclonal antibody to human apolipoprotein J. Folia Biol (Praha). 2002;48(1):40-2.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15(11):909-912.
PubMed
Variant
0.1 mg
11-421-C100
In stock

0.025 mg
11-421-C025
In stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock