Anti-SLP76 Purified

Anti-SLP76 Purified
Regulatory status
RUO
Antigen
SLP76
Clone
SLP-76/03
Format
Purified
Reactivity
Pig, Human, Mouse
Application
WB, IHC(P)
Variant
0.1 mg
11-402-C100
In stock
180.00 USD

0.025 mg
11-402-C025
Ask for delivery term HERE
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2b
Specificity
The monoclonal antibody reacts with SLP76, a 76kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils; in B cells, however, it is replaced by SLP65.
Application
WB, IHC(P)
Application details
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: thymus
Reactivity
Pig, Human, Mouse
Immunogen
Bacterially expressed fusion protein representing amino acids 216-434 of human SLP76 with histidine tag
Other names
SLP-76, LCP2
Concentration
1 mg/ml
Preparation
Purified by protein-G affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
SLP-76, LCP2
Antigen description
SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-gamma. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.
Entrez Gene ID 3937
UniProt ID Q13094
11-402 WB
Western blotting analysis (reducing conditions) of whole cell lysate using anti-SLP76 (SLP-76/03). Lane 1: HPB-ALL human peripheral blood T cell leukemia cell line Lane 2: JURKAT human peripheral blood T cell leukemia cell line Lane 3: RAMOS human Burkitt lymphoma cell line

General references:

Pivniouk VI, Martin TR, Lu-Kuo JM, Katz HR, Oettgen HC, Geha RS: SLP-76 deficiency impairs signaling via the high-affinity IgE receptor in mast cells. J Clin Invest. 1999 Jun;103(12):1737-43.
PubMed
Silverman MA, Shoag J, Wu J, Koretzky GA: Disruption of SLP-76 interaction with Gads inhibits dynamic clustering of SLP-76 and FcepsilonRI signaling in mast cells. Mol Cell Biol. 2006 Mar;26(5):1826-38.
PubMed
Koretzky GA, Abtahian F, Silverman MA: SLP76 and SLP65: complex regulation of signalling in lymphocytes and beyond. Nat Rev Immunol. 2006 Jan;6(1):67-78.
PubMed
Beach D, Gonen R, Bogin Y, Reischl IG, Yablonski D: Dual role of SLP-76 in mediating T cell receptor-induced activation of phospholipase C-gamma1. J Biol Chem. 2007 Feb 2;282(5):2937-46.
PubMed
Bogin Y, Ainey C, Beach D, Yablonski D: SLP-76 mediates and maintains activation of the Tec family kinase ITK via the T cell antigen receptor-induced association between SLP-76 and ITK. Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6638-43.
PubMed

Product specific references:

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15(11):909-912.
PubMed
Di Bartolo V, Montagne B, Salek M, Jungwirth B, Carrette F, Fourtane J, Sol-Foulon N, Michel F, Schwartz O, Lehmann WD, Acuto O: A novel pathway down-modulating T cell activation involves HPK-1-dependent recruitment of 14-3-3 proteins on SLP-76. J Exp Med. 2007 Mar 19;204(3):681-91
PubMed
Variant
0.1 mg
11-402-C100
In stock
180.00 USD

0.025 mg
11-402-C025
Ask for delivery term HERE
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD