Anti-SHIP1 Purified

Anti-SHIP1 Purified
Regulatory status
RUO
Antigen
SHIP1
Clone
SHIP-01
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-524-C100
In stock
150.00 USD

0.025 mg
11-524-C025
Delivery 1 week
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD

0.1 mg
11-261-C100
In stock
75.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2a
Specificity
The antibody SHIP-01 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells (intracellular antigen). Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.
Application
Application details
Flow cytometry: Intracellular staining; recommended dilution: 2-5 μg/ml; positive control: human blood leukocytes.
Western blotting: Positive control: RAMOS human cell line, reducing conditions.
Reactivity
Human
Immunogen
Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
IPP5PD, IP5PD, INPP5D
Antigen description
SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5´inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane.
Entrez Gene ID 3635
UniProt ID Q92835
11-524 WB
Western blotting analysis (reducing conditions) of human SHIP-1 in whole cell lysate of THP-1 human acute monocytic leukemia cell line. Lane 1: immunostaining with Isotype mouse IgG1 control (PPV-04; 11-458-C100) Lane 2,3: immunostaining with anti-human SHIP-1 (SHIP-01) Lane 4,5: immunostaining with anti-human SHIP-1 (SHIP-02; cat. no. 11-525-C100)

General references:

Harris SJ, Parry RV, Westwick J, Ward SG: Phosphoinositide lipid phosphatases: natural regulators of phosphoinositide 3-kinase signaling in T lymphocytes. J Biol Chem. 2008 Feb 1;283(5):2465-9.
PubMed
Xing W, Hamaguchi M: Effects of SHIP-1 on MMP2 secretion and invasion of SR3Y1 cells. J Genet Genomics. 2007 Apr;34(4):285-93.
PubMed
Tarasenko T, Kole HK, Chi AW, Mentink-Kane MM, Wynn TA, Bolland S: T cell-specific deletion of the inositol phosphatase SHIP reveals its role in regulating Th1/Th2 and cytotoxic responses. Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11382-7.
PubMed
Ai J, Maturu A, Johnson W, Wang Y, Marsh CB, Tridandapani S: The inositol phosphatase SHIP-2 down-regulates FcgammaR-mediated phagocytosis in murine macrophages independently of SHIP-1. Blood. 2006 Jan 15;107(2):813-20.
PubMed

Product specific references:

Lo TC, Barnhill LM, Kim Y, Nakae EA, Yu AL, Diccianni MB: Inactivation of SHIP1 in T-cell acute lymphoblastic leukemia due to mutation and extensive alternative splicing. Leuk Res. 2009 Nov;33(11):1562-6.
PubMed
Variant
0.1 mg
11-524-C100
In stock
150.00 USD

0.025 mg
11-524-C025
Delivery 1 week
75.00 USD
Variant
0.1 mg
11-261-C100
In stock
150.00 USD

0.1 mg
11-261-C100
In stock
75.00 USD

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