Exbio — Research products — Antibodies — Other intracellular antigens — Anti-R-RAS2 PE

Anti-R-RAS2 PE

Regulatory status

RUO

Antigen

R-RAS2

Clone

EM-50

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-722-C100

In stock

374.00 USD

0.025 mg

1P-722-C025

Delivery 1 week

187.00 USD

Variant

0.1 mg

1P-722-C100

In stock

374.00 USD

0.025 mg

1P-722-C025

Delivery 1 week

187.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody EM-50 recognizes R-Ras2 / TC21 protein (intracellular antigen) and does not react with R-Ras1, H-Ras, K-Ras, and N-Ras.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.

Reactivity

Human

Immunogen

Human TC21

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TC21, RRAS2

Antigen description

R-Ras2 / TC21 is the only member of R-Ras family of small GTPases that shows transforming activities similar to H-Ras, N-Ras, and K-Ras, and it is also structurally similar to them. R-Ras2 seems to play an important role in activating signal transduction pathways that control cell proliferation. Its mutations are associated with the growth of certain tumors, but also overexpression of the wild type form of R-Ras2 has been frequently detected in various carcinomas. Pseudogenes of R-Ras2 gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants.

Entrez Gene ID 22800

UniProt ID P62070

Separation of R-Ras2 transfected HEK-293 cells (red) from nontransfected HEK-293 cells (blue) in flow cytometry analysis (intracellular staining) stained using anti-R-RAS2 (EM-50) PE antibody (concentration in sample 1 μg/ml).

General references:

Gutierrez-Erlandsson S, Herrero-Vidal P, Fernandez-Alfara M, Hernandez-Garcia S, Gonzalo-Flores S, Mudarra-Rubio A, Fresno M, Cubelos B: R-RAS2 overexpression in tumors of the human central nervous system. Mol Cancer. 2013 Oct 23;12(1):127.
PubMed