Anti-PAG1 Purified

Anti-PAG1 Purified
Regulatory status
RUO
Antigen
PAG1
Clone
PAb (409)
Format
Purified
Reactivity
Mouse, Human
Application
Variant
0.1 mg
11-409-C100
In stock
180.00 USD

0.025 mg
11-409-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
Isotype
Rabbit polyclonal
Specificity
The polyclonal antibody recognizes intracellular part of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Application
Application details
Immunohistochemistry (paraffin sections): Positive tissue: tonsil, colon germinal center.
Western blotting: Recommended dilution: 1-2 μg/ml.
Reactivity
Mouse, Human
Immunogen
Recombinant intracellular fragment (aa 97-432) of human Cbp (PAG).
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CBP, PAG
Antigen description
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Entrez Gene ID 94212
UniProt ID Q3U1F9
11-409_WB
Western blotting analysis of human PAG using rabbit polyclonal antibody PAb (409) on lysates of Daudi and Ramos cell line under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rabbit anti-PAG monoclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. PAG was detected around 65 kDa.
Variant
0.1 mg
11-409-C100
In stock
180.00 USD

0.025 mg
11-409-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD