Exbio — Research products — Antibodies — Other intracellular antigens — Anti-LIME1 Purified

Anti-LIME1 Purified

Regulatory status

RUO

Antigen

LIME1

Clone

LIME-10

Format

Purified

Reactivity

Human

Application

FC, WB, IHC(P)

Variant

0.1 mg

11-401-C100

Ask for delivery term HERE

198.00 USD

0.025 mg

11-401-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-401-C100

Ask for delivery term HERE

198.00 USD

0.025 mg

11-401-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The antibody LIME-10 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Application

FC, WB, IHC(P)

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml.
Western blotting: Reducing conditions; recommended dilution: 1 μg/ml.
Flow cytometry: Recommended dilution: 1-5 μg/ml.

Reactivity

Human

Immunogen

COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

LITA1

Antigen description

LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.

Entrez Gene ID 54923

UniProt ID Q9H400

Western blotting analysis of human LIME using mouse monoclonal antibody LIME-10 on lysates of J77-LIME transfectants and Jurkat cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 1 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. LIME was detected around 30 kDa.

Anti-Hu LIME1 purified antibody (clone LIME-10) specificity verification by flow cytometry

Analysis of the antibody staining was performed on fixed and permeabilized (eBioscience™ Intracellular Fixation & Permeabilization Buffer Set, Thermofisher) cell suspension.
Anti-Hu LIME1 purified antibody (clone LIME-10) (concentration in sample 1 μg/ml, red-filled histogram) binds specifically to intacellular LIME1 in transfected cells (upper panel), but not to Jurkat cells (lower panel).
Level of non-specific binding was assessed using Mouse IgG1 isotype control purified antibody (MOPC-21) under same conditions (concentration in sample 1 μg/ml, black-dashed histogram).

General references:

Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16.
PubMed

Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17(1):35-49.
PubMed

Product specific references:

Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rudiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107(1):213-21.
PubMed

Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V. LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med. 2003 Nov 17;198(10):1453-62.
PubMed