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Exbio
—
Research products
—
Antibodies
—
Other intracellular antigens
—
Anti-Hu GAPDH Purified
Anti-Hu GAPDH Purified
Regulatory status
RUO
Antigen
GAPDH
Clone
FF26A
Format
Purified
Reactivity
Human
Application
IP, WB, IHC(F)
Variant
0.1 mg
11-942-C100
In stock
180.00 USD
Variant
0.1 mg
11-942-C100
In stock
180.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody FF26A recognizes human GAPDH (glyceraldehyne-3-phosphate dehydrogenase), a ubiquitously expressed 37 kDa enzyme involved in glycolysis and other processes.
Application
IP, WB, IHC(F)
Application details
Western blotting: 1 µg/ml; both reducing and non-reducing conditions; excellent with human antigen, negative results with murine and porcine material.
Reactivity
Human
Negative species
Mouse, Pig
Immunogen
human CD4-positive lymphocytes
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
G3PD, GAPD
Antigen description
GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is an intracellular homotetramerizing enzyme, which catalyzes oxidation of glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate during glycolysis, and has also functions in DNA replication, gene expression, and microtubule bundling. As it binds to many proteins, its functions are very broad. GAPDH plays also roles in cancer progression, apoptosis, Huntington disease and Alzheimer´s disease.
Entrez Gene ID
2597
UniProt ID
P04406
Anti-GAPDH Purified (FF26A) works in WB application under both reducing and non-reducing conditions.
Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of HeLa, HEK 293T, Jurkat and Raji cell lines mixed and heated (100°C, 5 min) with reducing (2-mercaptoethanol) or non-reducing SDS-loading buffer. Samples were resolved using 15% Tris-glycine SDS gel electrophoresis.
Nitrocellulose membrane blot was probed simultaneously with mouse IgM monoclonal antibody TU-10 (1 µg/ml) and mouse IgG1 anti-GAPDH monoclonal antibody FF26A (1 µg/ml). Subclass-specific secondary antibodies IRDye 680RD Goat-anti-Mouse IgM (red) and IRDye 800CW Goat-anti-Mouse IgG (green) were used for multiplex fluorescent Western blot detection.
GAPDH was detected at ~35 kDa in all tested cell lines.
Product specific references:
Liu Y, Cao J, Zhu YN, Ma Y, Murtaza G, Li Y, Wang JH, Pu YS: C1222C deletion in exon 8 of ABL1 is involved in carcinogenesis and cell cycle control of colorectal cancer through IRS1/PI3K/Akt pathway. Front Oncol. 2020 Aug 11;10:1385.
PubMed
Tarantelli C, Cannas E, Ekeh H, Moscatello C, Gaudio E, Cascione L, Napoli S, Rech C, Testa A, Maniaci C, Rinaldi A, Zucca E, Stathis A, Ciulli A, Bertoni F: The bromodomain and extra-terminal domain degrader MZ1 exhibits preclinical anti-tumoral activity in diffuse large B-cell lymphoma of the activated B cell-like type. Explor Target Antitumor Ther. 2021;2(6):586-601.
PubMed
Arkhypov I, Özbay Kurt FG, Bitsch R, Novak D, Petrova V, Lasser S, Hielscher T, Groth C, Lepper A, Hu X, Li W, Utikal J, Altevogt P, Umansky V: HSP90α induces immunosuppressive myeloid cells in melanoma via TLR4 signaling. J Immunother Cancer. 2022 Sep;10(9):e005551.
PubMed
Veggiani G, Villaseñor R, Martyn GD, Tang JQ, Krone MW, Gu J, Chen C, Waters ML, Pearce KH, Baubec T, Sidhu SS: High-affinity chromodomains engineered for improved detection of histone methylation and enhanced CRISPR-based gene repression. Nat Commun. 2022 Nov 30;13(1):7381.
PubMed
Liu CC, Leclair P, Pedari F, Vieira H, Monajemi M, Sly LM, Reid GS, Lim CJ: Integrins and ERp57 coordinate to regulate cell surface calreticulin in immunogenic cell death. Front Oncol. 2019 May 28;9:411.
PubMed
Fonseka CL, Hardman CS, Woo J, Singh R, Nahler J, Yang J, Chen YL, Kamaladasa A, Silva T, Salimi M, Gray N, Dong T, Malavige GN, Ogg GS: Dengue virus co-opts innate type 2 pathways to escape early control of viral replication. Commun Biol. 2022 Jul 22;5(1):735.
PubMed
Hartwig J, Loebel M, Steiner S, Bauer S, Karadeniz Z, Roeger C, Skurk C, Scheibenbogen C, Sotzny F: Metformin attenuates ROS via FOXO3 activation in immune cells. Front Immunol. 2021 Apr 19;12:581799.
PubMed
Triguero-Martínez A, Roy-Vallejo E, Montes N, de la Fuente H, Ortiz AM, Castañeda S, González-Álvaro I, Lamana A: Genetic LGALS1 variants are associated with heterogeneity in galectin-1 serum levels in patients with early arthritis. Int J Mol Sci. 2022 Jun 28;23(13):7181.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
11-942-C100
In stock
180.00 USD
Variant
0.1 mg
11-942-C100
In stock
180.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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