Exbio — Research products — Antibodies — Other intracellular antigens — Anti-Hu GAPDH Purified

Anti-Hu GAPDH Purified

Regulatory status

RUO

Antigen

GAPDH

Clone

FF26A

Format

Purified

Reactivity

Human

Application

IP, WB, IHC-F

Variant

0.1 mg

11-942-C100

In stock

203.50 USD

Variant

0.1 mg

11-942-C100

In stock

203.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody FF26A recognizes human GAPDH (glyceraldehyne-3-phosphate dehydrogenase), a ubiquitously expressed 37 kDa enzyme involved in glycolysis and other processes.

Application

IP, WB, IHC-F

Application details

Western blotting: 1 µg/ml; both reducing and non-reducing conditions; excellent with human antigen, negative results with murine and porcine material.

Reactivity

Human

Negative species

Mouse, Pig

Immunogen

human CD4-positive lymphocytes

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

G3PD, GAPD

Antigen description

GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is an intracellular homotetramerizing enzyme, which catalyzes oxidation of glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate during glycolysis, and has also functions in DNA replication, gene expression, and microtubule bundling. As it binds to many proteins, its functions are very broad. GAPDH plays also roles in cancer progression, apoptosis, Huntington disease and Alzheimer´s disease.

Entrez Gene ID 2597

UniProt ID P04406

Anti-GAPDH Purified (FF26A) works in WB application under both reducing and non-reducing conditions.

Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of HeLa, HEK 293T, Jurkat and Raji cell lines mixed and heated (100°C, 5 min) with reducing (2-mercaptoethanol) or non-reducing SDS-loading buffer. Samples were resolved using 15% SDS-PAGE gel.

Nitrocellulose membrane blot was probed simultaneously with mouse IgM monoclonal antibody TU-10 (1 µg/ml) and mouse IgG1 anti-GAPDH monoclonal antibody FF26A (1 µg/ml). Subclass-specific secondary antibodies IRDye 680RD Goat-anti-Mouse IgM (red) and IRDye 800CW Goat-anti-Mouse IgG (green) were used for multiplex fluorescent Western blot detection.

GAPDH was detected at ~35 kDa in all tested cell lines.

Product specific references:

Liu Y, Cao J, Zhu YN, Ma Y, Murtaza G, Li Y, Wang JH, Pu YS: C1222C deletion in exon 8 of ABL1 is involved in carcinogenesis and cell cycle control of colorectal cancer through IRS1/PI3K/Akt pathway. Front Oncol. 2020 Aug 11;10:1385.
PubMed

Tarantelli C, Cannas E, Ekeh H, Moscatello C, Gaudio E, Cascione L, Napoli S, Rech C, Testa A, Maniaci C, Rinaldi A, Zucca E, Stathis A, Ciulli A, Bertoni F: The bromodomain and extra-terminal domain degrader MZ1 exhibits preclinical anti-tumoral activity in diffuse large B-cell lymphoma of the activated B cell-like type. Explor Target Antitumor Ther. 2021;2(6):586-601.
PubMed

Arkhypov I, Özbay Kurt FG, Bitsch R, Novak D, Petrova V, Lasser S, Hielscher T, Groth C, Lepper A, Hu X, Li W, Utikal J, Altevogt P, Umansky V: HSP90α induces immunosuppressive myeloid cells in melanoma via TLR4 signaling. J Immunother Cancer. 2022 Sep;10(9):e005551.
PubMed

Veggiani G, Villaseñor R, Martyn GD, Tang JQ, Krone MW, Gu J, Chen C, Waters ML, Pearce KH, Baubec T, Sidhu SS: High-affinity chromodomains engineered for improved detection of histone methylation and enhanced CRISPR-based gene repression. Nat Commun. 2022 Nov 30;13(1):7381.
PubMed

Liu CC, Leclair P, Pedari F, Vieira H, Monajemi M, Sly LM, Reid GS, Lim CJ: Integrins and ERp57 coordinate to regulate cell surface calreticulin in immunogenic cell death. Front Oncol. 2019 May 28;9:411.
PubMed

Fonseka CL, Hardman CS, Woo J, Singh R, Nahler J, Yang J, Chen YL, Kamaladasa A, Silva T, Salimi M, Gray N, Dong T, Malavige GN, Ogg GS: Dengue virus co-opts innate type 2 pathways to escape early control of viral replication. Commun Biol. 2022 Jul 22;5(1):735.
PubMed

Hartwig J, Loebel M, Steiner S, Bauer S, Karadeniz Z, Roeger C, Skurk C, Scheibenbogen C, Sotzny F: Metformin attenuates ROS via FOXO3 activation in immune cells. Front Immunol. 2021 Apr 19;12:581799.
PubMed

Triguero-Martínez A, Roy-Vallejo E, Montes N, de la Fuente H, Ortiz AM, Castañeda S, González-Álvaro I, Lamana A: Genetic LGALS1 variants are associated with heterogeneity in galectin-1 serum levels in patients with early arthritis. Int J Mol Sci. 2022 Jun 28;23(13):7181.
PubMed