Anti-EGFR PE-Cy™7

Anti-EGFR PE-Cy™7
Regulatory status
RUO
Antigen
EGFR
Clone
EGFR1
Format
PE-Cy™7
Reactivity
Horse, Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T7-680-T100
In stock
275.00 USD

25 tests
T7-680-T025
In stock
137.50 USD
Variant
100 tests
T7-680-T100
In stock
275.00 USD

25 tests
T7-680-T025
In stock
137.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2b kappa
Specificity
The mouse monoclonal antibody EGFR1 reacts with extracellular domain of human protein kinase EGFR (ErbB1 / HER1); epitope within amino acids 6-273.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Horse, Human
Negative species
Mouse, Rat
Immunogen
Human epidermoid carcinoma line A431
Preparation
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
EGF-R, Her1, ERBB1, PIG61, NISBD2
Antigen description
The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.
Entrez Gene ID 1956
UniProt ID P00533
T7-680 FC
Separation of EGFR positive A431 cells (red-filled) from blank (FMO, black-dashed) in flow cytometry analysis (surface staining) stained using anti-EGFR (EGFR1) PE-Cy™7 antibody.

Product specific references:

Fortunel NO, Hatzfeld JA, Rosemary PA, Ferraris C, Monier MN, Haydont V, Longuet J, Brethon B, Lim B, Castiel I, Schmidt R, Hatzfeld A: Long-term expansion of human functional epidermal precursor cells: promotion of extensive amplification by low TGF-beta1 concentrations. J Cell Sci. 2003 Oct 1;116(Pt 19):4043-52.
PubMed
Aigner A, Juhl H, Malerczyk C, Tkybusch A, Benz CC, Czubayko F: Expression of a truncated 100 kDa HER2 splice variant acts as an endogenous inhibitor of tumour cell proliferation. Oncogene. 2001 Apr 19;20(17):2101-11.
PubMed
Akiyama M, Smith LT, Shimizu H: Changing patterns of localization of putative stem cells in developing human hair follicles. J Invest Dermatol. 2000 Feb;114(2):321-7.
PubMed
Rebetz J, Tian D, Persson A, Widegren B, Salford LG, Englund E, Gisselsson D, Fan X: Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma. PLoS One. 2008 Apr 9;3(4):e1936.
PubMed
Variant
100 tests
T7-680-T100
In stock
275.00 USD

25 tests
T7-680-T025
In stock
137.50 USD
Variant
100 tests
T7-680-T100
In stock
275.00 USD

25 tests
T7-680-T025
In stock
137.50 USD

Related products

-134%
Reg. statusCE IVD
ApplicationFC
100 ml
220.00 USD
-134%
Reg. statusCE IVD
ApplicationFC
100 ml
66.00 USD
-134%
Reg. statusRUO
ApplicationFC
100 ml
55.00 USD