Exbio — Research products — Antibodies — Other intracellular antigens — Anti-AGPS APC

Anti-AGPS APC

Regulatory status

RUO

Antigen

AGPS

Clone

AGPS-03

Format

APC

Reactivity

Human

Application

FC(IC)

Excitation laser

red (633 nm)

Variant

0.1 mg

1A-739-C100

In stock

242.00 USD

Variant

0.1 mg

1A-739-C100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody AGPS-03 recognizes AGPS (alkykglycerone phosphate synthase), an intracellular peroxisomal enzyme important for lipid biosynthesis.

Application

FC(IC)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Reactivity

Human

Immunogen

recombinant human AGPS (amino acids 158-384)

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ADAS, ADPS, RCDP3, ADAP-S, ADHAPS, ALDHPSY

Antigen description

AGPS (alkylglycerone phosphate synthase), is an enzyme that catalyzes the second step of ether lipid biosynthesis in which acyl-dihydroxyacetone phosphate (acyl-DHAP) is converted to alkyl-DHAP by addition of a long chain alcohol and removal of a long-chain acid anion. The protein is localized to the inner side of the peroxisomal membrane and requires FAD as a cofactor. Mutations in AGPS gene have been associated with type 3 of rhizomelic chondrodysplasia punctata (RCDP3), and Zellweger syndrome. Higher expression of AGPS was observed in BCR/ABL positive leukemias and it was also described to be associated with higher risk of relapse.

Entrez Gene ID 8540

UniProt ID O00116

Separation of A431 cells stained using anti-AGPS (MHD4-46) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from A431 cells stained using mouse IgG2a isotype control (MOPC-173) APC antibody (concentration in sample 5 μg/ml, same as AGPS APC antibody concentration, black-dashed) in flow cytometry analysis (intracellular staining).

General references:

Itzkovitz B, Jiralerspong S, Nimmo G, Loscalzo M, Horovitz DD, Snowden A, Moser A, Steinberg S, Braverman N: Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3. Hum Mutat. 2012 Jan;33(1):189-97
PubMed

Grimm MO, Kuchenbecker J, Rothhaar TL, Grösgen S, Hundsdörfer B, Burg VK, Friess P, Müller U, Grimm HS, Riemenschneider M, Hartmann T: Plasmalogen synthesis is regulated via alkyl-dihydroxyacetonephosphate-synthase by amyloid precursor protein processing and is affected in Alzheimer's disease. J Neurochem. 2011 Mar;116(5):916-25.
PubMed

Bhojwani D, Kang H, Menezes RX, Yang W, Sather H, Moskowitz NP, Min DJ, Potter JW, Harvey R, Hunger SP, Seibel N, Raetz EA, Pieters R, Horstmann MA, Relling MV, den Boer ML, Willman CL, Carroll WL: Gene expression signatures predictive of early response and outcome in high-risk childhood acute lymphoblastic leukemia: A children's oncology group study. J Clin Oncol. 2008 Sep 20;26(27):4376-84.
PubMed

Yeoh EJ, Ross ME, Shurtleff SA, Williams WK, Patel D, Mahfouz R, Behm FG, Raimondi SC, Relling MV, Patel A, Cheng C, Campana D, Wilkins D, Zhou X, Li J, Liu H, Pui CH, Evans WE, Naeve C, Wong L, Downing JR: Classification, subtype discovery, and prediction of outcome in pediatric acute lymphoblastic leukemia by gene expression profiling. Cancer Cell. 2002 Mar;1(2):133-43.
PubMed