Exbio — Research products — Antibodies — Nucleus — Anti-TIAR Purified

Anti-TIAR Purified

Regulatory status

RUO

Antigen

TIAR

Clone

6E3

Format

Purified

Reactivity

Human, Mouse, Non-human primates

Application

ELISA, WB, FC (QC tested), IHC-P, ICC

Variant

0.1 mg

11-920-C100

In stock

154.00 USD

Variant

0.1 mg

11-920-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody 6E3 recognizes TIAR, an ubiquitously expressed approximately 42 kDa intracellular RNA-binding protein.

Application

ELISA, WB, FC (QC tested), IHC-P, ICC

Application details

Flow cytometry: Intracellular staining; recommended dilution: 5 µg/ml

Reactivity

Human, Mouse, Non-human primates

Immunogen

GST-tagged recombinant human TIAR

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TCBP, TIAL1

Antigen description

TIAR is an ubiquitously expressed RNA-binding protein, which regulates translational control, splicing, and other activities, including apoptosis. TIAR attenuates CDK1 activity, and is essential for the G2/M checkpoint. It accumulates in nuclear foci in late G2 phase and prophase in cells under replication stress. In steady state TIAR shuttles between the cytoplasm and the nucleus, probably as a part of nucleocytoplasmic transport of mRNA, but under stress conditions it accumulates mRNA molecules in granules and prevents their translation. Nucleolytic activity of TIAR against attacked target cells of cytotoxic lymphocytes has also been reported. Similarly, e.g. in permeabilized thymocytes TIAR triggers DNA fragmentation.

Entrez Gene ID 7073

UniProt ID Q01085

Flow cytometry staining of TIAR in human cell line A-431 using purified mouse monoclonal antibody 6E3 (concentration in sample 5 μg/ml, GAM FITC, red-filled) from A-431 cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining).

Product specific references:

Beck AR, Miller IJ, Anderson P, Streuli M: RNA-binding protein TIAR is essential for primordial germ cell development. Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2331-6.
PubMed

Kedersha NL, Gupta M, Li W, Miller I, Anderson P: RNA-binding proteins TIA-1 and TIAR link the phosphorylation of eIF-2 alpha to the assembly of mammalian stress granules. J Cell Biol. 1999 Dec 27;147(7):1431-42.
PubMed

Piecyk M, Wax S, Beck AR, Kedersha N, Gupta M, Maritim B, Chen S, Gueydan C, Kruys V, Streuli M, Anderson P: TIA-1 is a translational silencer that selectively regulates the expression of TNF-alpha. EMBO J. 2000 Aug 1;19(15):4154-63.
PubMed

Shukla S, Dirksen WP, Joyce KM, Le Guiner-Blanvillain C, Breathnach R, Fisher SA: TIA proteins are necessary but not sufficient for the tissue-specific splicing of the myosin phosphatase targeting subunit 1. J Biol Chem. 2004 Apr 2;279(14):13668-76.
PubMed