Anti-TIAR Purified

Anti-TIAR Purified
Regulatory status
RUO
Antigen
TIAR
Clone
6E3
Format
Purified
Reactivity
Human, Mouse, Non-human primates
Variant
0.1 mg
11-920-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody 6E3 recognizes TIAR, an ubiquitously expressed approximately 42 kDa intracellular RNA-binding protein.
Application details
Flow cytometry: Intracellular staining; recommended dilution: 5 µg/ml
Reactivity
Human, Mouse, Non-human primates
Immunogen
GST-tagged recombinant human TIAR
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TCBP, TIAL1
Antigen description
TIAR is an ubiquitously expressed RNA-binding protein, which regulates translational control, splicing, and other activities, including apoptosis. TIAR attenuates CDK1 activity, and is essential for the G2/M checkpoint. It accumulates in nuclear foci in late G2 phase and prophase in cells under replication stress. In steady state TIAR shuttles between the cytoplasm and the nucleus, probably as a part of nucleocytoplasmic transport of mRNA, but under stress conditions it accumulates mRNA molecules in granules and prevents their translation. Nucleolytic activity of TIAR against attacked target cells of cytotoxic lymphocytes has also been reported. Similarly, e.g. in permeabilized thymocytes TIAR triggers DNA fragmentation.
Entrez Gene ID 7073
UniProt ID Q01085
11-920_FC_Histogram
Flow cytometry staining of TIAR in human cell line A-431 using purified mouse monoclonal antibody 6E3 (concentration in sample 5 μg/ml, GAM FITC, red-filled) from A-431 cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining).

Product specific references:

Beck AR, Miller IJ, Anderson P, Streuli M: RNA-binding protein TIAR is essential for primordial germ cell development. Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2331-6.
PubMed
Kedersha NL, Gupta M, Li W, Miller I, Anderson P: RNA-binding proteins TIA-1 and TIAR link the phosphorylation of eIF-2 alpha to the assembly of mammalian stress granules. J Cell Biol. 1999 Dec 27;147(7):1431-42.
PubMed
Piecyk M, Wax S, Beck AR, Kedersha N, Gupta M, Maritim B, Chen S, Gueydan C, Kruys V, Streuli M, Anderson P: TIA-1 is a translational silencer that selectively regulates the expression of TNF-alpha. EMBO J. 2000 Aug 1;19(15):4154-63.
PubMed
Shukla S, Dirksen WP, Joyce KM, Le Guiner-Blanvillain C, Breathnach R, Fisher SA: TIA proteins are necessary but not sufficient for the tissue-specific splicing of the myosin phosphatase targeting subunit 1. J Biol Chem. 2004 Apr 2;279(14):13668-76.
PubMed
Variant
0.1 mg
11-920-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD

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