Anti-STAT1 Purified

Anti-STAT1 Purified
Regulatory status
RUO
Antigen
STAT1
Clone
SM1
Format
Purified
Reactivity
Mouse, Human
Application
IP, WB, IHC(P)
Variant
0.1 mg
11-371-C100
In stock
180.00 USD

0.025 mg
11-371-C025
Out of stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Images
References
Isotype
Mouse IgG2b
Specificity
The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
Application
IP, WB, IHC(P)
Reactivity
Mouse, Human
Immunogen
STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE).
Other names
CANDF7, IMD31, ISGF3
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-371_WB
Detection of Human phosphorylated STAT1[p Ser727] and total STAT1 by Western Blot. Western blot shows lysate of human malignant melanoma cells (short-term culture derived from a patient) in response to interferon treatment to induce phosphorylation of STAT1 at Ser727. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Specific band was detected for phosphorylated STAT1[Ser727] by the antibody PSM1 (upper panel) and total STAT1 level by the antibody SM1 (lower panel).

General references:

Kovarik P, Stoiber D, Eyers PA, Menghini R, Neininger A, Gaestel M, Cohen P, Decker T: Stress-induced phosphorylation of STAT1 at Ser727 requires p38 mitogen-activated protein kinase whereas IFN-gamma uses a different signaling pathway. Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13956-61.
PubMed
Frank DA: STAT signaling in the pathogenesis and treatment of cancer. Mol Med. 1999 Jul;5(7):432-56.
PubMed
Bromberg J, Darnell JE Jr: The role of STATs in transcriptional control and their impact on cellular function. Oncogene. 2000 May 15;19(21):2468-73.
PubMed
Soond SM, Townsend PA, Barry SP, Knight RA, Latchman DS, Stephanou A: ERK and the E3 ubiquitin ligase beta TRCP targets STAT1 for degradation. J Biol Chem. 2008 Mar 31
PubMed

Product specific references:

Kovarik J, Boudny V, Kocak I, Lauerova L, Fait V, Vagundova M: Malignant melanoma associates with deficient IFN-induced STAT 1 phosphorylation. Int J Mol Med. 2003 Sep;12(3):335-40.
PubMed
Boudny V, Kocak I, Lauerova L, Kovarik J: Interferon inducibility of STAT 1 activation and its prognostic significance in melanoma patients. Folia Biol (Praha). 2003;49(4):142-6.
PubMed
Variant
0.1 mg
11-371-C100
In stock
180.00 USD

0.025 mg
11-371-C025
Out of stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD