Exbio — Research products — Antibodies — MHC antigens — Anti-HLA-G Purified

Anti-HLA-G Purified

Regulatory status

RUO

Antigen

HLA-G

Clone

MEM-G/2

Format

Purified

Reactivity

Human

Application

WB, IHC(F), IHC(P)

Variant

0.1 mg

11-436-C100

In stock

0.00 USD

0.025 mg

11-436-C025

In stock

0.00 USD

Variant

0.1 mg

11-261-C100

In stock

0.00 USD

0.1 mg

11-261-C100

In stock

0.00 USD

Contact distributor

Product details

Images

References

Isotype

Mouse IgG1

Specificity

The antibody MEM-G/2 recognizes an extracellular epitope on free heavy chain of all the HLA-G isoforms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.

Application

WB, IHC(F), IHC(P)

Reactivity

Human

Immunogen

Bacterially expressed recombinant HLA-G heavy chain (denatured).

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography

Formulation

Phosphate buffered saline (PBS) solution with 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Immunohistochemistry staining of kidney allograft biopsy (paraffin-embedded sections) with anti-human HLA-G (MEM-G/2).

Western blotting analysis of HLA-G in LCL-HLA-G transfectants using anti-HLA-G (MEM-G/2).

General references:

Creput C, Durrbach A, Menier C, Guettier C, Samuel D, Dausset J, Charpentier B, Carosella ED, Rouas-Freiss N. Human leukocyte antigen-G (HLA-G) expression in biliary epithelial cells is associated with allograft acceptance in liver-kidney transplantation. J Hepatol. 2003 39(4):587-94.
PubMed

Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, LeDanff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 64(3):315-26.
PubMed

Product specific references:

Le Discorde M, Moreau P, Sabatier P, Legeais JM, Carosella ED: Expression of HLA-G in human cornea, an immune-privileged tissue. Hum Immunol. 2003 Nov;64(11):1039-44.
PubMed

Poláková K, Bandzuchová E, Hofmeister V, Weiss EH, Hutter H, Russ G: Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients. Neoplasma. 2003;50(5):331-8.
PubMed

Lazana I, Zoudiari A, Kokkinou D, Themeli M, Liga M, Papadaki H, Papachristou D, Spyridonidis A: Identification of a novel HLA-G+ regulatory population in blood: expansion after allogeneic transplantation and de novo HLA-G expression at graft-versus-host disease sites. Haematologica. 2012 Sep;97(9):1338-47
PubMed

Boyson JE, Erskine R, Whitman MC, Chiu M, Lau JM, Koopman LA, Valter MM, Angelisova P, Horejsi V, Strominger JL: Disulfide bond-mediated dimerization of HLA-G on the cell surface. Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16180-5.
PubMed

Variant

0.1 mg

11-436-C100

In stock

0.00 USD

0.025 mg

11-436-C025

In stock

0.00 USD

Variant

0.1 mg

11-261-C100

In stock

0.00 USD

0.1 mg

11-261-C100

In stock

0.00 USD

Contact distributor

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