Anti-HLA-G FITC

Anti-HLA-G FITC
Regulatory status
RUO
Antigen
HLA-G
Clone
01G
Format
FITC
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1F-438-C100
In stock
260.00 USD

0.025 mg
1F-438-C025
Delivery 1 week
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
260.00 USD

0.1 mg
11-261-C100
In stock
130.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody 01G recognizes membrane-bound form of HLA-G (full-length HLA-G1), but not soluble forms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Application
Application details
Flow cytometry: Recommended dilution: 5 μg/ml.
Reactivity
Human
Negative species
Mouse, Rat
Immunogen
HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
Concentration
1 mg/ml
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Entrez Gene ID 3135
UniProt ID P17693
1F-438 FC
Separation of HLA-G transfected LCL cells (red) from K562 cells (black) in flow cytometry analysis (surface staining) stained using anti-HLA-G (01G) FITC antibody (concentration in sample 27 μg/ml).

General references:

Creput C, Durrbach A, Menier C, Guettier C, Samuel D, Dausset J, Charpentier B, Carosella ED, Rouas-Freiss N. Human leukocyte antigen-G (HLA-G) expression in biliary epithelial cells is associated with allograft acceptance in liver-kidney transplantation. J Hepatol. 2003 39(4):587-94.
PubMed
Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, LeDanff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 64(3):315-26.
PubMed

Product specific references:

Polakova K, Russ G: Expression of the non-classical HLA-G antigen in tumor cell lines is extremely restricted. Neoplasma. 2000;47(6):342-8.
PubMed
Polakova K, Bandzuchova E, Hofmeister V, Weiss EH, Hutter H, Russ G: Binding analysis of HLA-G specific antibodies to hematopoietic cells isolated from leukemia patients. Neoplasma. 2003;50(5):331-8.
PubMed
Polakova K, Krcova M, Kuba D, Russ G: Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients. Leuk Res. 2003 Jul;27(7):643-8.
PubMed
Wiendl H, Feger U, Mittelbronn M, Jack C, Schreiner B, Stadelmann C, Antel J, Brueck W, Meyermann R, Bar-Or A, Kieseier BC, Weller M: Expression of the immune-tolerogenic major histocompatibility molecule HLA-G in multiple sclerosis: implications for CNS immunity. Brain. 2005 Nov;128(Pt 11):2689-2704.
PubMed
Real LM, Cabrera T, Collado A, Jimenez P, Garcia A, Ruiz-Cabello F, Garrido F: Expression of HLA G in human tumors is not a frequent event. Int J Cancer. 1999 May 17;81(4):512-8.
PubMed
Real LM, Cabrera T, Canton J, Oliva R, Ruiz-Cabello F, Garrido F: Looking for HLA-G expression in human tumours. J Reprod Immunol. 1999 Jul;43(2):263-73.
PubMed
Variant
0.1 mg
1F-438-C100
In stock
260.00 USD

0.025 mg
1F-438-C025
Delivery 1 week
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
260.00 USD

0.1 mg
11-261-C100
In stock
130.00 USD

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