Exbio — Research products — Antibodies — MHC antigens — Anti-HLA-DR Purified

Anti-HLA-DR Purified

Regulatory status

RUO

Antigen

HLA-DR

Clone

MEM-12

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested)

Variant

0.1 mg

11-474-C100

In stock

154.00 USD

0.025 mg

11-474-C025

Delivery 1 week

77.00 USD

Variant

0.1 mg

11-474-C100

In stock

154.00 USD

0.025 mg

11-474-C025

Delivery 1 week

77.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-12 recognizes a common extracellular epitope on human HLA-DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).

Application

IP, WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 2 μg/ml; positive control: B-lymphocytes, monocytes, DAUDI cell line, RAJI cell line.
Western blotting and Immunoprecipitation: Positive control: B-lymphocytes, monocytes, DAUDI cell line, RAJI cell line; non-reducing conditions.

Reactivity

Human

Immunogen

thymocyte membrane

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR (MEM-12) purified antibody (concentration in sample 0,3 μg/ml, GAM APC).

Separation of human monocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human HLA-DR (MEM-12) purified antibody (concentration in sample 0,3 μg/ml, GAM APC).

Product specific references:

Horejsi V, Nemec M, Angelisova P, Kristofova H, Gorga JC, Hilgert I: Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens. 1986 Nov;28(5):288-97.
PubMed

Sochorová K, Budínský V, Rožková D, Tobiášová Z, Dusilová-Sulková S, Spíšek R, Bartůňková J: Paricalcitol (19-nor-1,25-dihydroxyvitamin D2) and calcitriol (1,25-dihydroxyvitamin D3) exert potent immunomodulatory effects on dendritic cells and inhibit induction of antigen-specific T cells. Clin Immunol. 2009 Oct;133(1):69-77.
PubMed