Exbio — Research products — Antibodies — MHC antigens — Anti-HLA Class I Purified

Anti-HLA Class I Purified

Regulatory status

RUO

Antigen

HLA Class I

Clone

MEM-147

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested)

Variant

0.1 mg

11-242-C100

In stock

154.00 USD

0.025 mg

11-242-C025

Delivery 1 week

77.00 USD

Variant

0.1 mg

11-242-C100

In stock

154.00 USD

0.025 mg

11-242-C025

Delivery 1 week

77.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-147 reacts with an extracellular epitope of all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.

Application

IP, WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.
Western blotting: Non-reducing conditions.

Reactivity

Human

Immunogen

PHA-activated peripheral blood lymphocytes

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Western blotting analysis of human Ramos B cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb MEM-147 and goat anti-mouse IgG (H+L)-HRP conjugate.

Separation of human leukocytes stained using anti-HLA Class I (MEM-147) purified antibody (concentration in sample 1.67 μg/ml, GAM PE, red-filled) from human leukocytes unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (surface staining).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-HLA Class I (MEM-147) purified antibody (concentration in sample 1.67 μg/ml) GAM PE.

Product specific references:

Ilangumaran S, Briol A, Hoessli DC: CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes. Blood. 1998 May 15;91(10):3901-8.
PubMed

Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84.
PubMed

Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics. 2001 Aug;53(6):440-6.
PubMed