Exbio — Research products — Antibodies — MHC antigens — Anti-HLA-C PE

Anti-HLA-C PE

Regulatory status

RUO

Antigen

HLA-C

Clone

DT-9

Format

Reactivity

Non-human primates, Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-896-T100

In stock

242.00 USD

Variant

100 tests

1P-896-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody DT-9 recognizes an extracellular epitope on HLA-C member of MHC class I molecules. It does not crossreact with HLA-A or HLA-B allotypes.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Non-human primates, Human

Immunogen

Purified MHC class I molecules of tamarin origin

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

HLC-C, D6S204, PSORS1, HLA-JY3

Antigen description

HLA-C, a member of MHC class I glycoproteins, is one of polymorphysm typing targets, which are important for transplantation. The HLA system plays an important role in the occurrence and outcome of infectious diseases. The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19), are reported to contain multiple Class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population. It has been described that HLA-C interacts with human herpesvirus 8 MIR1 protein.

Entrez Gene ID 3107

UniProt ID P10321

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-C (DT-9) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human basophils (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-C (DT-9) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Körner C, Simoneau CR, Schommers P, Granoff M, Ziegler M, Hölzemer A, Lunemann S, Chukwukelu J, Corleis B, Naranbhai V, Kwon DS, Scully EP, Jost S, Kirchhoff F, Carrington M, Altfeld M: HIV-1-Mediated Downmodulation of HLA-C Impacts Target Cell Recognition and Antiviral Activity of NK Cells. Cell Host Microbe 2017 Jul 12;22(1):111-119.
PubMed

Tremante E, Lo Monaco E, Ingegnere T, Sampaoli C, Fraioli R, Giacomini P: Monoclonal antibodies to HLA-E bind epitopes carried by unfolded β2 m-free heavy chains . Eur J Immunol. 2015 Aug;45(8):2356-64.
PubMed

Serena M, Parolini F, Biswas P, Sironi F, Blanco Miranda A, Zoratti E, Scupoli MT, Ziglio S, Valenzuela-Fernandez A, Gibellini D, Romanelli MG, Siccardi A, Malnati M, Beretta A, Zipeto D: HIV-1 Env associates with HLA-C free-chains at the cell membrane modulating viral infectivity. Sci Rep. 2018 May 22;8:46991. doi: 10.1038/srep46991.
PubMed

Corrah TW, Goonetilleke N, Kopycinski J, Deeks SG, Cohen MS, Borrow P, McMichael A, Brackenridge S: Reappraisal of the relationship between the HIV-1-protective single-nucleotide polymorphism 35 kilobases upstream of the HLA-C gene and surface HLA-C expression. J Virol. 2011 Apr;85(7):3367-74.
PubMed

van der Ploeg K, Chang C, Ivarsson MA, Moffett A, Wills MR, Trowsdale J: Modulation of human leukocyte antigen-C by human cytomegalovirus stimulates KIR2DS1 recognition by natural killer cells. Front Immunol. 2017 Mar 29;8:298.
PubMed

Parolini F, Biswas P, Serena M, Sironi F, Muraro V, Guizzardi E, Cazzoletti L, Scupoli MT, Gibellini D, Ugolotti E, Biassoni R, Beretta A, Malnati M, Romanelli MG, Zipeto D: Stability and expression levels of HLA-C on the cell membrane modulate HIV-1 infectivity. J Virol. 2017 Dec 14;92(1):e01711-17.
PubMed