Exbio — Research products — Antibodies — Isotype controls — Mouse IgG3 Isotype Control DyLight<sup>®</sup> 488

Mouse IgG3 Isotype Control DyLight^® 488

Regulatory status

RUO

Clone

PPV-07

Format

DyLight^® 488

Application

WB, FC (QC tested), IHC-F, IHC-P, ICC

Excitation laser

blue (488 nm)

Variant

0.1 mg

L4-459-C100

In stock

209.00 USD

Variant

0.1 mg

L4-459-C100

In stock

209.00 USD

Contact distributor

Product details

Images

SDS download

Isotype

Mouse IgG3

Specificity

This mouse IgG3 monoclonal antibody (clone PPV-07) reacts with undefined epitope on a plant pathogen.

Application

WB, FC (QC tested), IHC-F, IHC-P, ICC

Application details

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

Negative species

Mouse, Rat, Pig, Human

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with DyLight^® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

DyLight^® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG3 Isotype control (PPV-07) DyLight® 488 antibody (concentration in sample 3 μg/ml).