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Exbio
—
Research products
—
Antibodies
—
Cytoskeleton and ECM
—
Anti-Neurofilament H Purified
Anti-Neurofilament H Purified
Regulatory status
RUO
Antigen
Neurofilament H
Clone
NF-05
Format
Purified
Reactivity
Human, Rat, Pig
Application
ELISA, WB, IHC(F)
Variant
0.1 mg
11-440-C100
In stock
160.00 USD
0.025 mg
11-440-C025
Out of stock
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD
0.1 mg
11-261-C100
In stock
80.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody NF-05 recognizes a nonphosphorylated epitope of neurofilament heavy protein (NF-H), a 210 kDa intracellular structural protein of Intermediate Filament Proteins family. NF-H is mainly expressed in the central and peripheral nervous system and reproductive system and is biochemically very stable.
Application
ELISA, WB, IHC(F)
Application details
ELISA: Capture antibody.
Western blotting: Recommended dilution: 1 μg/ml; positive control: lysate of brain homogenate.
Reactivity
Human, Rat, Pig
Negative species
Mouse
Immunogen
Pig brain neurofilament protein-enriched fraction after depolymerization of microtubules
Other names
NFH, NEFH, Neurofilament heavy chain, CMT2CC
Concentration
1 mg/ml
Preparation
Purified by precipitation and chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Western blotting analysis of neurofilament heavy protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-05.
General references:
Cote F, Collard JF, Julien JP: Progressive neuronopathy in transgenic mice expressing the human neurofilament heavy gene: a mouse model of amyotrophic lateral sclerosis. Cell. 1993 Apr 9;73(1):35-46.
PubMed
Ohara O, Gahara Y, Miyake T, Teraoka H, Kitamura T: Neurofilament deficiency in quail caused by nonsense mutation in neurofilament-L gene. J Cell Biol. 1993 Apr;121(2):387-95.
PubMed
Brown RH: Amyotrophic lateral sclerosis: recent insights from genetics and transgenic mice. Cell. 1995 Mar 10;80(5):687-92.
Wild EJ, Petzold A, Keir G, Tabrizi SJ: Plasma neurofilament heavy chain levels in Huntington's disease. Neurosci Lett. 2007 May 7;417(3):231-3.
PubMed
Miyazawa I, Nakashima I, Petzold A, Fujihara K, Sato S, Itoyama Y: High CSF neurofilament heavy chain levels in neuromyelitis optica. Neurology. 2007 Mar 13;68(11):865-7.
PubMed
Petzold A, Keir G, Warren J, Fox N, Rossor MN: A systematic review and meta-analysis of CSF neurofilament protein levels as biomarkers in dementia. Neurodegener Dis. 2007;4(2-3):185-94.
PubMed
Product specific references:
Porchet R, Probst A, Draberova E, Draber P, Riederer IM, Riederer BM: Differential subcellular localization of phosphorylated neurofilament and tau proteins in degenerating neurons of the human entorhinal cortex. Neuroreport. 2003 May 23;14(7):929-33.
PubMed
Variant
0.1 mg
11-440-C100
In stock
160.00 USD
0.025 mg
11-440-C025
Out of stock
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD
0.1 mg
11-261-C100
In stock
80.00 USD
Contact distributor
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