Anti-Drebrin PE

Anti-Drebrin PE
Regulatory status
RUO
Antigen
Drebrin
Clone
DBN-N-03
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1P-694-T100
Out of stock

25 tests
1P-694-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
References
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody DBN-N-03 recognizes drebrin, an approximately 100-125 kDa regulator of actin cytoskeleton.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Bacterially expressed N-terminal fragment of recombinant human drebrin (aa 1-326)
Other names
DBN, D0S117E
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

General references:

Pérez-Martínez M, Gordón-Alonso M, Cabrero JR, Barrero-Villar M, Rey M, Mittelbrunn M, Lamana A, Morlino G, Calabia C, Yamazaki H, Shirao T, Vázquez J, González-Amaro R, Veiga E, Sánchez-Madrid F: F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. J Cell Sci. 2010 Apr 1;123(Pt 7):1160-70.
PubMed
Hayashi K, Ishikawa R, Kawai-Hirai R, Takagi T, Taketomi A, Shirao T: Domain analysis of the actin-binding and actin-remodeling activities of drebrin. Exp Cell Res. 1999 Dec 15;253(2):673-80.
PubMed
Hayashi K, Ishikawa R, Ye LH, He XL, Takata K, Kohama K, Shirao T: Modulatory role of drebrin on the cytoskeleton within dendritic spines in the rat cerebral cortex. J Neurosci. 1996 Nov 15;16(22):7161-70.
PubMed
Sasaki Y, Hayashi K, Shirao T, Ishikawa R, Kohama K: Inhibition by drebrin of the actin-bundling activity of brain fascin, a protein localized in filopodia of growth cones. J Neurochem. 1996 Mar;66(3):980-8.
PubMed
Ishikawa R, Hayashi K, Shirao T, Xue Y, Takagi T, Sasaki Y, Kohama K: Drebrin, a development-associated brain protein from rat embryo, causes the dissociation of tropomyosin from actin filaments. J Biol Chem. 1994 Nov 25;269(47):29928-33.
PubMed

Product specific references:

Vaskova M, Kovac M, Volna P, Angelisova P, Mejstrikova E, Zuna J, Brdicka T, Hrusak O: High expression of cytoskeletal protein drebrin in TEL/AML1pos B-cell precursor acute lymphoblastic leukemia identified by a novel monoclonal antibody. Leuk Res. 2011 Aug;35(8):1111-3. Epub 2011 Apr 16.
PubMed
Variant
100 tests
1P-694-T100
Out of stock

25 tests
1P-694-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock