Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Cytokeratins Purified

Anti-Cytokeratins Purified

Regulatory status

RUO

Antigen

Cytokeratins

Clone

C-11

Format

Purified

Reactivity

Mammalian

Application

FC-IC, IP, WB, IHC-P, ICC

Variant

0.1 mg

11-108-C100

In stock

143.00 USD

0.025 mg

11-108-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-108-C100

In stock

143.00 USD

0.025 mg

11-108-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.

Application

FC-IC, IP, WB, IHC-P, ICC

Application details

Flow cytometry: Recommended dilution: 1 μg/ml. Intracellular staining.
Immunohistochemistry: Recommended dilution: 2-8 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml.

Reactivity

Mammalian

Immunogen

Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

cytokeratin, CYK, CK, KRT

Antigen description

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Immunocytochemistry staining of cytokeratins in Hep-2 cells using pan-cytokeratin antibody C-11 (11-108-C100, diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green).

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Anti-Hu Cytokeratins purified antibody (clone C-11) specificity verification by flow cytometry

Anti-Cytokeratins (C-11) purified antibody (concentration in sample 0.3 μg/ml, GAM APC, red-filled histogram) binds specifically to intracellular cytokeratins in permeabilized HeLa cells (upper panel), but does not stain permeabilized TF-1 cells (lower panel). Level of non-specific binding was assessed using cells unstained by primary antibody (GAM APC, black-dashed histogram).

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with HeLa cells stained using anti-Cytokeratins (C-11) purified antibody (GAM APC, concentration in sample 0.3 μg/ml).

Immunohistochemistry staining of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody (C-11).

Western blotting analysis of human cytokeratins using mouse monoclonal antibody C-11 on lysates of HeLa cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-cytokeratins followed by IRDye800-conjugated anti-mouse secondary antibody. Specific bands were detected for cytokeratins at approximately 45-55 kDa.

Anti-Hu Cytokeratins Purified (clone C-11) reactivity pattern in WB application.
The reactivity of C-11 antibody was assessed by comparing binding signals in a panel of cytokeratin-positive and cytokeratin-negative human cell lines. Western blotting analysis was performed on whole cell extracts (hot 1% SDS lysis buffer) of HeLa, MCF-7, Jurkat, and Molt-4 cell lines, mixed and heated (100°C, 5 min) with reducing (2-mercaptoethanol) SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody C-11 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). For multiplex fluorescent Western blot detection, mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml) was used as the loading control (red).

Cytokeratins were detected at ~38-55 kDa in the respective cell lines.

Product specific references:

Hamakawa H, Sumida T, Tanioka H, Sogawa K, Yamada T: Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis. Res Commun Mol Pathol Pharmacol. 1998 Aug;101(2):115-26.
PubMed

Broekema M, Harmsen MC, Koerts JA, Petersen AH, van Luyn MJ, Navis G, Popa ER: Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats. Kidney Int. 2005 Dec;68(6):2572-81.
PubMed

Vojtesek B, Stasková Z, Nenutil R, Lauerová L, Kovarík J, Rejthar A, Bártková J, Bártek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82.
PubMed

Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5.
PubMed

Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24.
PubMed