Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Cytokeratin 19 Purified

Anti-Cytokeratin 19 Purified

Regulatory status

RUO

Antigen

Cytokeratin 19

Clone

BA-17

Format

Purified

Reactivity

Human

Application

FC(IC), IP, WB, IHC(P), ICC

Variant

0.1 mg

11-119-C100

In stock

154.00 USD

0.025 mg

11-119-C025

Delivery 1 week

77.00 USD

Variant

0.1 mg

11-119-C100

In stock

154.00 USD

0.025 mg

11-119-C025

Delivery 1 week

77.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The antibody BA-17 reacts with cytokeratin 19 (40 kDa intracellular antigen) in human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.

Application

FC(IC), IP, WB, IHC(P), ICC

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Immunohistochemistry: Recommended dilution: 5-10 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml.

Reactivity

Human

Immunogen

Mammary organoids.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

K19, CK19, CYK19, KRT19, K1CS

Antigen description

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Entrez Gene ID 3880

UniProt ID P08727

Immunohistochemistry staining (paraffin sections) of cytokeratin 19 in human liver using mouse monoclonal antibody BA-17 (11-119-C100, diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1µg/ml; orange).

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (BA-17) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 19 (BA-17). Commercially tested by LifeSpan BioSciences.

Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody BA-17 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 19 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for cytokeratin 19 at approximately 41 kDa.

Product specific references:

Bartek J, Taylor-Papadimitriou J, Miller N, Millis R: Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours. Int J Cancer. 1985 Sep 15;36(3):299-306.
PubMed

Bartek J, Durban EM, Hallowes RC, Taylor-Papadimitriou J: A subclass of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins. J Cell Sci. 1985 Apr;75:17-33.
PubMed

Lewis BC, Klimstra DS, Varmus HE: The c-myc and PyMT oncogenes induce different tumor types in a somatic mouse model for pancreatic cancer. Genes Dev. 2003 Dec 15;17(24):3127-38. Epub 2003 Dec 17.
PubMed