Anti-Cytokeratin 19 Purified

Anti-Cytokeratin 19 Purified
Regulatory status
RUO
Antigen
Cytokeratin 19
Clone
BA-17
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-119-C100
In stock
140.00 USD

0.025 mg
11-119-C025
Delivery 1 week
70.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD

0.1 mg
11-261-C100
In stock
70.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody BA-17 reacts with cytokeratin 19 (40 kDa intracellular antigen) in human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Immunohistochemistry: Recommended dilution: 5-10 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml.
Reactivity
Human
Immunogen
Mammary organoids.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
K19, CK19, CYK19, KRT19, K1CS
Antigen description
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Entrez Gene ID 3880
UniProt ID P08727
11-119_IHC
Immunohistochemistry staining (paraffin sections) of cytokeratin 19 in human liver using mouse monoclonal antibody BA-17 (11-119-C100, diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1µg/ml; orange).
11-119_IHC
Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 19 (BA-17). Commercially tested by LifeSpan BioSciences.
11-119_WB
Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody BA-17 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 19 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for cytokeratin 19 at approximately 41 kDa.

Product specific references:

Bartek J, Taylor-Papadimitriou J, Miller N, Millis R: Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours. Int J Cancer. 1985 Sep 15;36(3):299-306.
PubMed
Bartek J, Durban EM, Hallowes RC, Taylor-Papadimitriou J: A subclass of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins. J Cell Sci. 1985 Apr;75:17-33.
PubMed
Lewis BC, Klimstra DS, Varmus HE: The c-myc and PyMT oncogenes induce different tumor types in a somatic mouse model for pancreatic cancer. Genes Dev. 2003 Dec 15;17(24):3127-38. Epub 2003 Dec 17.
PubMed
Variant
0.1 mg
11-119-C100
In stock
140.00 USD

0.025 mg
11-119-C025
Delivery 1 week
70.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD

0.1 mg
11-261-C100
In stock
70.00 USD

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