Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Actin (muscle) Purified

Anti-Actin (muscle) Purified

Regulatory status

RUO

Antigen

Actin (muscle)

Clone

HHF35

Format

Purified

Reactivity

Human, Chicken, Mouse, Non-human primates, Dog, Rat, Rabbit, Cat

Application

WB, IHC(F), IHC(P), ICC

Variant

0.1 mg

11-600-C100

In stock

180.00 USD

0.025 mg

11-600-C025

Delivery 1 week

90.00 USD

Variant

0.1 mg

11-600-C100

In stock

180.00 USD

0.025 mg

11-600-C025

Delivery 1 week

90.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.

Application

WB, IHC(F), IHC(P), ICC

Application details

Western blotting: Recommended dilution: 1 μg/ml; positive control: murine femoral muscle, murine heart, negative control: HUVEC line; reducing conditions.
Immunohistochemistry (paraffin sections): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended.

Reactivity

Human, Chicken, Mouse, Non-human primates, Dog, Rat, Rabbit, Cat

Immunogen

SDS extracted protein fraction of human myocardium

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Muscle specific actin

Antigen description

Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.

Immunohistochemistry staining of human muscle (paraffin-embedded sections) with anti-muscle-specific actin (HHF35). Commercially tested by LifeSpan BioSciences.

Western blotting analysis of muscle-specific actin using the monoclonal antibody HHF35. Lane 1: murine femoral muscle Lane 2: murine heart Lane 3: HeLa Lane 4: HUVEC

Product specific references:

Matsuyama A, Hisaoka M, Hashimoto H: Angioleiomyoma: a clinicopathologic and immunohistochemical reappraisal with special reference to the correlation with myopericytoma. Hum Pathol. 2007 Apr;38(4):645-51.
PubMed

Matsuo S, Susumu S, Tsutsumi R, Azuma T, Obata S, Hayashi T: Glomus tumor of the omentum: a case report. J Surg Oncol. 2007 Dec 1;96(7):633-6.
PubMed

Tsukada T, Tippens D, Gordon D, Ross R, Gown AM: HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization. Am J Pathol. 1987 Jan;126(1):51-60.
PubMed

Tsukada T, McNutt MA, Ross R, Gown AM: HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues. Am J Pathol. 1987 May;127(2):389-402.
PubMed

Schmidt RA, Cone R, Haas JE, Gown AM: Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins. Am J Pathol. 1988 Apr;131(1):19-28.
PubMed

Miettinen M: Antibody specific to muscle actins in the diagnosis and classification of soft tissue tumors. Am J Pathol. 1988 Jan;130(1):205-15.
PubMed

de Sousa SO, Schwarzschild M, de Araújo NS, de Araújo VC: Basal cell adenocarcinoma of the palate with squamous metaplasia. J Clin Pathol. 2000 Feb;53(2):153-6.
PubMed

Handharyani E, Ochiai K, Iwata N, Umemura T: Immunohistochemical and ultrastructural study of ito cells (fat-storing cells) in response to extrahepatic bile duct ligation in broiler chickens. J Vet Med Sci. 2001 May;63(5):547-52.
PubMed

Ijzer J, Roskams T, Molenbeek RF, Ultee T, Penning LC, Rothuizen J, van den Ingh TS: Morphological characterisation of portal myofibroblasts and hepatic stellate cells in the normal dog liver. Comp Hepatol. 2006 Nov 16;5:7.
PubMed