Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD92 PE

Anti-Hu CD92 PE

Regulatory status

RUO

Antigen

CD92

Clone

VIM15

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-866-T100

Ask for delivery term HERE

242.00 USD

Variant

100 tests

1P-866-T100

Ask for delivery term HERE

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody VIM15 recognizes an extracellular epitope of CD92, a 70 kDa transmembrane glycoprotein expressed mainly on human peripheral blood monocytes and neutrophils, and several myeloid and T-cell lines.

Workshop

HLDA VI: M-MR6, HLDA V: BP-BP229, M-M15

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

MV4-11 acute myeloid leukemia cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CTL1, CHTL1, SLC44A1

Antigen description

CD92 is a 70 kDa protein with ten transmembrane domains, intracellular N and C teminus, and two glycosylated larger extracellular loops. In the C-terminal domain, there is an ITIM-like sequence. This protein seems to be a choline transporter responsible for delivery of choline into the immune cells, to make it accessible for phospholipid synthesis, as well as a regulator of immune cell signaling. It is expressed mainly on human peripheral blood monocytes and neutrophils, and several myeloid and T-cell lines. It can also be found on mast cells (but not eosinophils), and weakly on peripheral blood lymphocytes, fibroblasts, epithelial cells, and endothelial cells.

Entrez Gene ID 23446

UniProt ID Q8WWI5

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD92 (VIM15) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD92 positive monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD92 (VIM15) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

Wille S, Szekeres A, Majdic O, Prager E, Staffler G, Stöckl J, Kunthalert D, Prieschl EE, Baumruker T, Burtscher H, Zlabinger GJ, Knapp W, Stockinger H: Characterization of CDw92 as a member of the choline transporter-like protein family regulated specifically on dendritic cells. J Immunol. 2001 Nov 15;167(10):5795-804.
PubMed