Anti-Hu CD43 Alexa Fluor® 700

Anti-Hu CD43 Alexa Fluor<sup>®</sup> 700
Regulatory status
RUO
Antigen
CD43
Clone
MEM-59
Format
Alexa Fluor® 700
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
100 tests
A7-220-T100
In stock
242.00 USD

25 tests
A7-220-T025
Delivery 1 week
121.00 USD
Variant
100 tests
A7-220-T100
In stock
242.00 USD

25 tests
A7-220-T025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody MEM-59 recognizes a neuraminidase-sensitive extracellular epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.
Workshop
HLDA IV: WS Code NL 604; HLDA V: WS Code AS S290
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human T lymphocytes.
Preparation
Purified antibody is conjugated with Alexa Fluor® 700 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
Leukosialin, Sialophorin, Galactoglycoprotein, GALGP, LSN, SPN, GALGP, GP5
Antigen description
CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.
Entrez Gene ID 6693
UniProt ID P16150
A7-220 FC
Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD43 (MEM-59) Alexa Fluor® 700.

General references:

Kadaja L, Laos S, Maimets T: Overexpression of leukocyte marker CD43 causes activation of the tumor suppressor proteins p53 and ARF. Oncogene. 2004 Apr 1;23(14):2523-30.
PubMed
Khunkaewla P, Schiller HB, Paster W, Leksa V, Cermák L, Anděra L, Hořejší V, Stockinger H: LFA-1-mediated leukocyte adhesion regulated by interaction of CD43 with LFA-1 and CD147. Mol Immunol. 2007 Nov 8
PubMed
Kadaja-Saarepuu L, Laos S, Jääger K, Viil J, Balikova A, Lõoke M, Hansson GC, Maimets T: CD43 promotes cell growth and helps to evade FAS-mediated apoptosis in non-hematopoietic cancer cells lacking the tumor suppressors p53 or ARF. Oncogene. 2007 Sep 24
PubMed
Mody PD, Cannon JL, Bandukwala HS, Blaine KM, Schilling AB, Swier K, Sperling AI: Signaling through CD43 regulates CD4 T-cell trafficking. Blood. 2007 Oct 15;110(8):2974-82.
PubMed
Stefanova I, Hilgert I, Kristofova H, Brown R, Low MG, Horejsi V: Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol. 1989 nFeb;26(2):153-61.
PubMed

Product specific references:

Schatzlmaier P, Supper V, Göschl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11
PubMed
Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry. J Immunol Methods. 2007 Jan 30;319(1-2):21-33.
PubMed
Cermak L, Simova S, Pintzas A, Horejsi V, Andera L: Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem. 2002 Mar 8;277(10):7955-61.
PubMed
Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar;13(3):423-36.
PubMed
Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
Alvarado M, Klassen C, Cerny J, Horejsi V, Schmidt RE: MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur J Immunol. 1995 Apr;25(4):1051-5.
PubMed
Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
Stefanova I, Hilgert I, Angelisova P, Kristofova H, Horejsi V: Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin. Folia Biol (Praha). 1988;34(4):255-65.
PubMed
Variant
100 tests
A7-220-T100
In stock
242.00 USD

25 tests
A7-220-T025
Delivery 1 week
121.00 USD
Variant
100 tests
A7-220-T100
In stock
242.00 USD

25 tests
A7-220-T025
Delivery 1 week
121.00 USD

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