Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD34 Purified Azide Free

Anti-Hu CD34 Purified Azide Free

Regulatory status

RUO

Antigen

CD34

Clone

QBEnd-10

Format

Purified Azide Free

Reactivity

Non-human primates, Human

Application

IP, WB, FC (QC tested), IHC(F), IHC(P), FUNC

Variant

0.1 mg

10-566-C100

In stock

143.00 USD

0.025 mg

10-566-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

10-566-C100

In stock

143.00 USD

0.025 mg

10-566-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody QBEnd-10 reacts with an extracellular class II epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.

Workshop

HLDA V: WS Code BP275, E038, MA065, MR09

Application

IP, WB, FC (QC tested), IHC(F), IHC(P), FUNC

Application details

Functional application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line.
Flow cytometry: Recommended dilution: 5 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 2-8 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml, positive control: TF-1 cells.

Reactivity

Non-human primates, Human

Negative species

Dog, Sheep, Rat, Cow

Immunogen

Human endothelial vesicles

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

Entrez Gene ID 947

UniProt ID P28906

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD34 (QBEnd-10) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

Flow cytometry multicolor surface staining of human peripheral blood stained using anti-human CD34 (QBEnd-10) purified antibody (concentration in sample 0,6 μg/ml, GAM APC, red-filled) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD45dim CD34 positive stem cells (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD34 (QBEnd-10) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

Western blotting analysis of human CD34 using mouse monoclonal antibody QBEnd-10 on lysates of TF-1 cell line and HEK293T/17 cell line (CD34 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD34 monoclonal antibody QBEnd-10 followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for CD34 protein at approximately 110 kDa.

Flow cytometry analysis (surface staining) of CD34 in human peripheral blood with anti-CD34 (QBEnd-10) azide free.

General references:

Krauter J, Hartl M, Hambach L, Kohlenberg A, Gunsilius E, Ganser A, Heil G: Receptor-mediated endocytosis of CD34 on hematopoietic cells after stimulation with the monoclonal antibody anti-HPCA-1. J Hematother Stem Cell Res. 2001 Dec;10(6):863-71.
PubMed

Dao MA, Arevalo J, Nolta JA: Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution. Blood. 2003 Jan 1;101(1):112-8.
PubMed

Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Gulati S, Luthra PM: Three-dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk-L. Stem Cells Dev. 2005 Oct;14(5):470-7.
PubMed

Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Luthra PM: Hematopoietic stem cell antigen CD34: role in adhesion or homing. Stem Cells Dev. 2006 Jun;15(3):305-13.
PubMed

Product specific references:

Schlingemann RO, Rietveld FJ, de Waal RM, Bradley NJ, Skene AI, Davies AJ, Greaves MF, Denekamp J, Ruiter DJ: Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma. Lab Invest. 1990 Jun;62(6):690-6.
PubMed

Ramani P, Bradley NJ, Fletcher CD: QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections. Histopathology. 1990 Sep;17(3):237-42.
PubMed

Kuzu I, Bicknell R, Harris AL, Jones M, Gatter KC, Mason DY: Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours. J Clin Pathol. 1992 Feb;45(2):143-8.
PubMed

Sutherland DR, Marsh JC, Davidson J, Baker MA, Keating A, Mellors A: Differential sensitivity of CD34 epitopes to cleavage by Pasteurella haemolytica glycoprotease: implications for purification of CD34-positive progenitor cells. Exp Hematol. 1992 Jun;20(5):590-9.
PubMed

Grimsley PG, Amos TA, Gordon MY, Greaves MF: Rapid positive selection of CD34+ cells using magnetic microspheres coated with monoclonal antibody QBEND/10 linked via a cleavable disulphide bond. Leukemia. 1993 Jun;7(6):898-908.
PubMed

Poblet E, Jimenez-Acosta F, Rocamora A: QBEND/10 (anti-CD34 antibody) in external root sheath cells and follicular tumors. J Cutan Pathol. 1994 Jun;21(3):224-8.
PubMed

Traoré Y, Hirn J: Certain anti-CD34 monoclonal antibodies induce homotypic adhesion of leukemic cell lines in a CD18-dependent and a CD18-independent way. Eur J Immunol. 1994 Oct;24(10):2304-11.
PubMed

Koumarianou A, Economopoulou P, Katsaounis P, Laschos K, Arapantoni-Dadioti P, Martikos G, Rogdakis A, Tzanakis N, Boukovinas I: Gastrointestinal stromal tumors (GIST): A prospective analysis and an update on biomarkers and current treatment concepts. Biomark Cancer 2015 May 21;7(Suppl 1):1-7.
PubMed

Castrechini NM, Murthi P, Gude NM, Erwich JJ, Gronthos S, Zannettino A, Brennecke SP, Kalionis B: Placenta. Mesenchymal stem cells in human placental chorionic villi reside in a vascular niche. 2010 Mar;31(3):203-12.
PubMed

Bonelli P, Nicolussi P, Manetti R, Antuofermo E, Dattena M: Monoclonal antigen do not cross-react with ovine umbilical cord blood cells. Ital J Anim Sci 2010, vol.9:e6.
PubMed