Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD315 PE

Anti-Hu CD315 PE

Regulatory status

RUO

Antigen

CD315

Clone

1F11

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-939-T100

In stock

242.00 USD

Variant

100 tests

1P-939-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 1F11 recognizes an extracellular epitope of CD315, a type I transmembrane glycoprotein expressed on keratinocytes, activated monocytes, and a subset of B cells.

Workshop

HCDM listed

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

HeLa cells

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

PF2RI, SMAP-6, PTGFRN, FPRP, EWI-F

Antigen description

CD315, also known as prostaglandin F2 receptor negative regulator, is an approximately 135 kDa transmembrane glycoprotein, which associates with actin cytoskeleton, and with CD9 and CD81, but not with other tetraspanins. It seems to be involved in regulation of cell polarity and motility. CD315 is expressed mainly by keratinocytes, activated monocytes, and a subset of B cells, but it can be also used for distinguishing between strongly positive colon cancer and fibrosarcoma cells, and their negative normal cell counterparts.

Entrez Gene ID 5738

UniProt ID Q9P2B2

Separation of A431 cells stained using anti-human CD315 (1F11) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from A431 cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as CD315 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of A431 cell suspension.

Product specific references:

Chambrion C, Le Naour F: The tetraspanins CD9 and CD81 regulate CD9P1-induced effects on cell migration. PLoS One 2010 Jun 21;5(6):e11219.
PubMed

André M, Chambrion C, Charrin S, Soave S, Chaker J, Boucheix C, Rubinstein E, Le Naour F: In situ chemical cross-linking on living cells reveals CD9P-1 cis-oligomer at cell surface. J Proteomics 2009 Nov 2;73(1):93-102.
PubMed

Charrin S, Manié S, Billard M, Ashman L, Gerlier D, Boucheix C, Rubinstein E: Multiple levels of interactions within the tetraspanin web. Biochem Biophys Res Commun. 2003 Apr 25;304(1):107-12.
PubMed

Charrin S, Le Naour F, Oualid M, Billard M, Faure G, Hanash SM, Boucheix C, Rubinstein E: The major CD9 and CD81 molecular partner. Identification and characterization of the complexes, J Biol Chem. 2001 Apr 27;276(17):14329-37.
PubMed