Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD31 Purified

Anti-Hu CD31 Purified

Regulatory status

RUO

Antigen

CD31

Clone

MEM-05

Format

Purified

Reactivity

Human

Application

WB, FC (QC tested), IHC(P), ICC

Variant

0.1 mg

11-273-C100

In stock

143.00 USD

0.025 mg

11-273-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-273-C100

In stock

143.00 USD

0.025 mg

11-273-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-05 reacts with an extracellular epitope of CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.

Application

WB, FC (QC tested), IHC(P), ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.
Western blotting: Non-reducing conditions.

Reactivity

Human

Immunogen

Leukocytes of a patient suffering from LGL-type leukaemia

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

PECAM-1, EndoCAM, GPIIA, PECA1

Antigen description

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

Entrez Gene ID 5175

UniProt ID P16284

Flow cytometry analysis (surface staining) of lysed and wash human peripheral blood with purified anti-human CD31 (MEM-05) (detection by Goat anti-mouse IgG1 PE).

General references:

Wong MX, Hayball JD, Jackson DE: PECAM-1-regulated signalling thresholds control tolerance in anergic transgenic B-cells. Mol Immunol. 2007 Oct 29
PubMed

Woodfin A, Voisin MB, Nourshargh S: PECAM-1: a multi-functional molecule in inflammation and vascular biology. Arterioscler Thromb Vasc Biol. 2007 Dec;27(12):2514-23.
PubMed

Wee JL, Jackson DE: The Ig-ITIM superfamily member PECAM-1 regulates the "outside-in" signaling properties of integrin alpha(IIb)beta3 in platelets. Blood. 2005 Dec 1;106(12):3816-23.
PubMed

Jackson DE: The unfolding tale of PECAM-1. FEBS Lett. 2003 Apr 10;540(1-3):7-14.
PubMed

Wilkinson R, Lyons AB, Roberts D, Wong MX, Bartley PA, Jackson DE: Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) acts as a regulator of B-cell development, B-cell antigen receptor (BCR)-mediated activation, and autoimmune disease. Blood. 2002 Jul 1;100(1):184-93.
PubMed

Newman DK, Hamilton C, Newman PJ: Inhibition of antigen-receptor signaling by Platelet Endothelial Cell Adhesion Molecule-1 (CD31) requires functional ITIMs, SHP-2, and p56(lck). Blood. 2001 Apr 15;97(8):2351-7.
PubMed

Product specific references:

Prager E, Staffler G, Majdic O, Saemann M, Godar S, Zlabinger G, Stockinger H: Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells. J Immunol. 2001 Feb 15;166(4):2364-71.
PubMed

Cârţână T, Săftoiu A, Gruionu LG, Gheonea DI, Pirici D, Georgescu CV, Ciocâlteu A, Gruionu G: Confocal laser endomicroscopy for the morphometric evaluation of microvessels in human colorectal cancer using targeted anti-CD31 antibodies. PLoS One. 2012;7(12):e52815. doi: 10.1371/journal.pone.0052815.
PubMed

Kalina T, Bakardjieva M, Blom M, Perez-Andres M, Barendregt B, Kanderová V, Bonroy C, Philippé J, Blanco E, Pico-Knijnenburg I, Paping JH, Wolska-Kuśnierz B, Pac M, Tkazcyk J, Haerynck F, Akar HH, Formánková R, Freiberger T, Svatoň M, Šedivá A, Arriba-Méndez S, Orfao A, van Dongen JJ, van der Burg M: EuroFlow standardized approach to diagnostic immunopheneotyping of severe PID innNewborns and young children. Front Immunol. 2020 Mar 19;11:371.
PubMed

Wei F, Cao S, Ren X, Liu H, Yu J, Li H, Hao X: Efficient antiproliferative and antiangiogenic effects on human ovarian cancer growth by gene transfer of attenuated mutants of Shiga-like toxin I. Int J Gynecol Cancer. Jul-Aug 2008;18(4):677-91.
PubMed