Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD303 PE

Anti-Hu CD303 PE

Regulatory status

RUO

Antigen

CD303

Clone

15E3

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-943-T100

In stock

242.00 USD

Variant

100 tests

1P-943-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 15E3 recognizes an extracellular epitope of human CD303, a transmembrane glycoprotein expressed on plasmacytoid dendritic cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

CD303 ectodomain fused with human IgG Fc domain

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

BDCA2, CLEC4C, DLEC, HECL, CLECSF7, CLECSF11, PRO34150

Antigen description

CD303 is an approximately 38 kDa type II transmembrane glycoprotein with an extracellular C-type lectin domain. It is a specific marker of plasmacytoid dendritic cells, and plays roles in capturing of pathogen-related oligosaccharide-containing antigens by them, and in their presentation to T cells. CD303 also mediates a potent inhibition of interferon alpha/beta production in plasmacytoid dendritic cells, thus it represents a potential target for lupus erythematosus therapy.

Entrez Gene ID 170482

UniProt ID Q8WTT0

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD303 (15E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining pattern of human peripheral blood mononuclear cells using anti-human CD303 (15E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD123 (6H6) APC antibody (10 μl reagent per milion cells in 100 μl of cell suspension).

Separation of human CD123 positive CD303 positive plasmacytoid dendritic cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD303 (15E3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).