Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD1d Purified

Anti-Hu CD1d Purified

Regulatory status

RUO

Antigen

CD1d

Clone

51.1

Format

Purified

Reactivity

Human, Non-human primates

Application

IP, WB, FC (QC tested), IHC(F)

Variant

0.1 mg

11-924-C100

In stock

154.00 USD

Variant

0.1 mg

11-924-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody 51.1 recognizes an extracellular epitope of CD1d, a 38 kDa transmembrane glycoprotein expressed mainly on cortical thymocytes, marginal zone B cells and other antigen presenting cells, but also i e.g. hepatitis C virus-infected livers.

Application

IP, WB, FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 4-8 μg/ml.

Reactivity

Human, Non-human primates

Immunogen

human CD1d

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

CD1d belongs to CD1 family of transmembrane glycoproteins, associated with beta2 microglobulin, similarly to MHC I molecules. Unlike other CD1 family members, however, CD1d can be also expressed in a non-glycosylated form, which is not associated with beta2 microglobulin. Hence it is not certain how much CD1d plays a role in the presentation of microbial lipid antigens during infection. On the other hand, it is expressed on various antigen presenting cell types. Besides it, CD1d+ thymocytes are involved in the positive selection of sublineage of NKT cells.

Entrez Gene ID 912

UniProt ID P15813

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD1d (51.1) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Separation of human CD1d positive lymphocytes (red-filled) from human CD1d negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD1d (51.1) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Product specific references:

Kim HS, Garcia J, Exley M, Johnson KW, Balk SP, Blumberg RS: Biochemical characterization of CD1d expression in the absence of beta2-microglobulin. J Biol Chem. 1999 Apr 2;274(14):9289-95.
PubMed

Durante-Mangoni E, Wang RJ, Shaulov A, He Q, Nasser I, Afdhal N, Koziel MJ, Exley MA: Hepatic CD1d expression in hepatitis C virus infection and recognition by resident proinflammatory CD1d-reactive T cells. J Immunol. 2004 Aug 1;173(3):2159-66.
PubMed