Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD187 PE

Anti-Hu CD187 PE

Regulatory status

RUO

Antigen

CD187

Clone

10D1-J16

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-879-T100

Ask for delivery term HERE

242.00 USD

Variant

100 tests

1P-879-T100

Ask for delivery term HERE

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody 10D1-J16 recognizes an extracellular epitope on CD187/CXCR7, a transmembrane protein of chemokine receptor family.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CXCR7, RDC1, GPR159, ACKR3

Antigen description

CD187 (CXCR7) is a member of chemokine receptor family, but with discussed specificity. It is expressed in various tissues and cells, such as placenta, urinary bladder, fetal liver cells, tumor cells, activated endothelium, monocytes, lymphocytes, mature dendritic cells, and other.

Entrez Gene ID 57007

UniProt ID P25106

Separation of MCF-7 cells stained using anti-human CD187 (10D1-J16) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from MCF-7 cells stained using mouse IgG2a isotype control (MOPC-173) PE antibody (concentration in sample 5 μg/ml, same as CD187 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of MCF-7 cell suspension.

Product specific references:

Doijen J, Van Loy T, De Haes W, Landuyt B, Luyten W, Schoofs L, Schols D: Signaling properties of the human chemokine receptors CXCR4 and CXCR7 by cellular electric impedance measurements. PLoS One. 2017 Sep 25;12(9):e0185354.
PubMed

Espinoza-Sánchez NA, Vadillo E, Balandrán JC, Monroy-García A, Pelayo R, Fuentes-Pananá EM: Evidence of lateral transmission of aggressive features between different types of breast cancer cells. Int J Oncol. 2017 Nov;51(5):1482-1496.
PubMed

Guan L, Li X, Wei J, Liang Z, Yang J, Weng X, Wu X: Antigen-specific CD8+ memory stem T cells generated from human peripheral blood effectively eradicate allogeneic targets in mice. Stem Cell Res Ther. 2018 Dec 7;9(1):337.
PubMed