Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD177 Purified

Anti-Hu CD177 Purified

Regulatory status

RUO

Antigen

CD177

Clone

MEM-166

Format

Purified

Reactivity

Non-human primates, Human

Application

IP, WB, FC (QC tested)

Variant

0.1 mg

11-314-C100

In stock

165.00 USD

0.025 mg

11-314-C025

Delivery 1 week

82.50 USD

Variant

0.1 mg

11-314-C100

In stock

165.00 USD

0.025 mg

11-314-C025

Delivery 1 week

82.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.

Workshop

HLDA VI: WS Code M M17; HLDA VI: WS Code BP 309

Application

IP, WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1 μg/ml.
Western blotting: Non-reducing conditions.

Reactivity

Non-human primates, Human

Immunogen

Human granulocytes

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

NB1, PRV1, HNA2A

Antigen description

CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).

Entrez Gene ID 57126

UniProt ID Q8N6Q3

Flow cytometry (surface staining) of human peripheral blood cells with anti-CD177 (MEM-166) purified, GAM-APC.

General references:

Bauer S, Abdgawad M, Gunnarsson L, Segelmark M, Tapper H, Hellmark T: Proteinase 3 and CD177 are expressed on the plasma membrane of the same subset of neutrophils. J Leukoc Biol. 2007 Feb;81(2):458-64.
PubMed

Sachs UJ, Andrei-Selmer CL, Maniar A, Weiss T, Paddock C, Orlova VV, Choi EY, Newman PJ, Preissner KT, Chavakis T, Santoso S: The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31). J Biol Chem. 2007 Aug 10;282(32):23603-12.
PubMed

Mnjoyan Z, Li J, Afshar-Kharghan V: Expression of polycythemia rubra vera-1 decreases the dependency of cells on growth factors for proliferation. Haematologica. 2005 Mar;90(3):405-6.
PubMed

Stroncek DF, Caruccio L, Bettinotti M: CD177: A member of the Ly-6 gene superfamily involved with neutrophil proliferation and polycythemia vera. J Transl Med. 2004 Mar 29;2(1):8.
PubMed

Leukocyte Typing VII., Mason D. et al. (Eds.), Oxford University Press (2002).