Anti-Hu CD164 PE

Anti-Hu CD164 PE
Regulatory status
RUO
Antigen
CD164
Clone
67D2
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1P-135-T100
In stock
Variant
0.1 mg
11-261-C100
In stock
Product details
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 67D2 recognizes class III epitope (not sensitive to sialidase, N-glycanase, O-glycosidase, and O-sialoglycoprotease) of CD164, a sialomucin expressed in hematopoietic myeloid and erythroid progenitors, activated basophils, and in various carcinomas and leukemic cells.
Workshop
HLDA VIII
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Breast tumor cell line T-47D
Other names
DFNA66, MUC-24, MGC-24
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

Product specific references:

Doyonnas R, Yi-Hsin Chan J, Butler LH, Rappold I, Lee-Prudhoe JE, Zannettino AC, Simmons PJ, Bühring HJ, Levesque JP, Watt SM: CD164 monoclonal antibodies that block hemopoietic progenitor cell adhesion and proliferation interact with the first mucin domain of the CD164 receptor. J Immunol. 2000 Jul 15;165(2):840-51.
PubMed
Jorgensen-Tye B, Levesque JP, Royle L, Doyonnas R, Chan JY, Dwek RA, Rudd PM, Harvey DJ, Simmons PJ, Watt SM: Epitope recognition of antibodies that define the sialomucin, endolyn (CD164), a negative regulator of haematopoiesis. Tissue Antigens. 2005 Mar;65(3):220-39.
PubMed
Pilling D, Vakil V, Gomer RH: Improved serum-free culture conditions for the differentiation of human and murine fibrocytes. J Immunol Methods. 2009 Dec 31;351(1-2):62-70.
PubMed
Hennersdorf F, Florian S, Jakob A, Baumgärtner K, Sonneck K, Nordheim A, Biedermann T, Valent P, Bühring HJ: Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation. Cell Res. 2005 May;15(5):325-35.
PubMed
Variant
100 tests
1P-135-T100
In stock
Variant
0.1 mg
11-261-C100
In stock