Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD151 PE

Anti-Hu CD151 PE

Regulatory status

RUO

Antigen

CD151

Clone

50-6

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-149-T100

Ask for delivery term HERE

242.00 USD

25 tests

1P-149-T025

Delivery 1 week

121.00 USD

Variant

100 tests

1P-149-T100

Ask for delivery term HERE

242.00 USD

25 tests

1P-149-T025

Delivery 1 week

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody CD151 recognizes an extracellular epitope of CD151 (also known as PETA-3), a 29 kDa transmembrane protein of tetraspanin family, expressed in many cell types.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Human epidermoid carcinoma cell line Hep-3

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

RAPH blood group, MER2, SFA1, PETA-3, GP27

Antigen description

CD151, also known as PETA-3 (platelet-endothelial tetraspan antigen), is a four-pass transmembrane glycoprotein with short cytoplasmic N- and C-termini. CD151 is expressed mainly in platelets and megakaryocytes, immature hematopoietic cells, activated T cells, in endothelium, muscle cells, and epithelial cells. It associates with CD9, CD181, and integrin complexes alpha 3 / beta 1 (CD49c / CD29), alpha 5 / beta 1 (CD49e / CD29), and alpha 6 / beta 4 (CD49f / CD104). CD151 appears to be involved in cell adhesion and migration, including metastasis.

Entrez Gene ID 977

UniProt ID P48509

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD151 (50-6) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD45 negative CD151 positive thrombocytes (red-filled) from CD151 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD151 (50-6) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Sadej R, Grudowska A, Turczyk L, Kordek R, Romanska HM: CD151 in cancer progression and metastasis: a complex scenario. Lab Invest. 2014 Jan;94(1):41-51.
PubMed

Spring FA, Griffiths RE, Mankelow TJ, Agnew C, Parsons SF, Chasis JA, Anstee DJ: Tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1. PLoS One. 2013 May 21;8(5):e62654.
PubMed