Exbio — Research products — Antibodies — CD and related antigens — Anti-Bov CD45R Alexa Fluor<sup>®</sup> 488

Anti-Bov CD45R Alexa Fluor^® 488

Regulatory status

RUO

Antigen

CD45R

Clone

IVA103

Format

Alexa Fluor^® 488

Reactivity

Cow

Application

FC (QC tested), ICC

Excitation laser

blue (488 nm)

Variant

0.1 mg

A4-352-C100

In stock

242.00 USD

Variant

0.1 mg

A4-352-C100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody IVA103 reacts with an extracellular epitope of CD45R antigen (bovine), a single chain type I transmembrane glycoprotein (member of the protein tyrosine phosphatase family), a restricted form of the CD45 family. CD45R is an essential regulator of T and B lymphocytes antigen receptor signaling and it is specifically expressed in hematopoietic cells.

Application

FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Cow

Immunogen

Bovine leukocytes.

Concentration

0.5 mg/ml

Preparation

Purified antibody is conjugated with Alexa Fluor^® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

PTPRCR, T200R

Antigen description

CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.

Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD45R (IVA103) Alexa Fluor® 488 antibody (concentration in sample 3 μg/ml).

Separation of bovine CD45R positive lymphocytes (red-filled) from CD45R negative cells (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD45R (IVA103) Alexa Fluor® 488 antibody (concentration in sample 3 μg/ml).

Product specific references:

Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18(8):365-8.
PubMed

Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996).
PubMed

Sopp P, Howard CJ: Investigation of PC36 (BoCD45R). Vet Immunol Immunopathol. 1996 Aug;52(4):271-4.
PubMed