SingleFlowEx MPO APC

SingleFlowEx MPO APC
Regulatory status
RUO (GMP)
Antigen
Myeloperoxidase
Clone
MPO421-8B2
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
1 ml
ED7624
Delivery 1 week
440.00 USD
Variant
0.1 mg
11-261-C100
In stock
440.00 USD
Product details
Images
References
Isotype
Mouse IgG1
Specificity
Myeloperoxidase
Application
Reactivity
Human
Description
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
Concentration
0.100 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
ED7624_FC_Histogram
Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx Myeloperoxidase APC
Clone: HM57
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; intracellular staining
Erythrocyte lysis and permeabilization: EXCELLYSE XPerm (ED7397) Positive events: Neutrophil granulocytes
Negative events: Lymphocytes
ED7624_FC_pacient
A - Flow cytometry intracellular staining pattern of human peripherial whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) APC antibody (10 μl reagent / 100 μl blood sample). Black - EDTA-anticoagulated human peripheral blood, healthy donor; Blue - Heparin-anticoagulated human peripheral blood, patient with MPO deficiency; Red - Heparin-anticoagulated human peripheral blood, mother of patient with MPO deficiency. B - Separation of human Myeloperoxidase positive neutrophil granulocytes of healthy donor (black-filled), patient with MPO deficiency (blue-filled) and mother of patient with MPO deficiency (red-filled) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
ED7624_FC_Profil Značení
Example of intracellular staining pattern using SingleFlowEx Myeloperoxidase APC
Clone: HM57
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; intracellular staining
Erythrocyte lysis and permeabilization: EXCELLYSE XPerm (ED7397)

Product specific references:

Use of this product has not been published.
Variant
1 ml
ED7624
Delivery 1 week
440.00 USD
Variant
0.1 mg
11-261-C100
In stock
440.00 USD

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