CD16 PE

CD16 PE
Regulatory status
CE IVD
Antigen
CD16
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
ED7016
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
References
Application
FC (QC tested)
Reactivity
Human
Description
The reagent CD16 PE permits identification and enumeration of cell populations expressing human CD16 antigen in whole blood using flow cytometry.
Contents
The reagent contains mouse monoclonal antibody against human CD16 antigen (clone LNK16) which was purified by affinity chromatography and labeled with R-Phycoerythrin (PE). The labeled antibody is diluted at optimum concentration in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide. The content of a vial (2 ml) is sufficient for 100 tests.
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended for In Vitro Diagnostic Use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Product specific references:

Boyle JJ (2004) Human macrophages kill human mesangial cells by Fas-L-induced apoptosis when triggered by antibody via CD16. Clin Exp Immunol. 137: 529-37
Arase N et al. (2003) IgE-mediated activation of NK cells through Fc gamma RIII. J Immunol. 170: 3054-8
Kocher M et al. (1997) Cross-linking of Fc gamma receptor IIa and Fc gamma receptor IIIb induces different proadhesive phenotypes on human neutrophils. J Immunol. 159: 3940-8
Tamm A and Schmidt RE (1996) The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding. J Immunol. 157: 1576-81
Gessner JE et al. (1995) The human low affinity immunoglobulin G Fc receptor III-A and III-B genes. Molecular characterization of the promoter regions. J Biol Chem. 270: 1350-61
Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Variant
100 tests
ED7016
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD