rDer f 2 (Dermatophagoides farinae 2.0116)

rDer f 2 (Dermatophagoides farinae 2.0116)
Regulatory status
RUO
Format
Purified Azide Free
Application
Variant
0.1 mg
10-P197-C100
In stock
242.00 USD
Variant
0.1 mg
10-P197-C100
In stock
242.00 USD
Product details
Images
SDS download
Application
Description
Recombinant protein rDer f 2 is expressed in S2 cells (Drosophila). DNA sequence encoding 129 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 15,6 kDa.
Contents
Recombinant protein rDer f 2 - 1 vial (0.1 mg), lyophilized.
Reconstitute with sterile deionized water with or without azide.
Preparation
Purified by sequential steps of ion exchange and affinity chromatography. Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0).
Storage and handling
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
10-P197_čistota
Recombinant allergen rDer f 2 purity verification. 5 µg of rDer f 2 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
10-P197_WB_Ponceau
Reactivity of rDer f 2 with sIgE antibodies in plasma of a patient with confirmed presence (routine clinical test) of specific IgE antibodies to Dermatophagoides farinae allergen extract.

Western blotting analysis was performed on recombinant allergen rDer f 2 mixed and heated (100°C, 5 min) with reducing SDS loading buffer. Sample was resolved using 15% Tris-glycine gel electrophoresis. Nitrocellulose membrane blot was stained with Ponceau S to verify protein transfer. After thorough washing and blocking steps, the membrane was incubated with plasma of an allergic patient (1:10). Captured rDer f 2 sIgE antibodies were detected by biotinylated anti-human IgE secondary antibody (2 μg/ml) followed by IRDye 800CW Streptavidin (1:5000).
Variant
0.1 mg
10-P197-C100
In stock
242.00 USD
Variant
0.1 mg
10-P197-C100
In stock
242.00 USD

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