Exbio — Research products — Recombinant allergens — Food — rAra h 8 (Arachis hypogaea 8.0101)

rAra h 8 (Arachis hypogaea 8.0101)

Regulatory status

RUO

Format

Purified Azide Free

Application

ELISA, WB

Variant

0.1 mg

10-P210-C100

In stock

242.00 USD

Variant

0.1 mg

10-P210-C100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

SDS download

Application

ELISA, WB

Description

Recombinant protein rAra h 8 is expressed in Escherichia coli. DNA sequence encoding 169 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 18,3 kDa.

Contents

Recombinant protein rAra h 8 - 1 vial (0.1 mg), lyophilized.
Reconstitute with sterile deionized water with or without azide.

Preparation

Purified by sequential steps of ion exchange and affinity chromatography. Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0).

Storage and handling

Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.

Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

UniProt ID Q6VT83

Recombinant allergen rAra h 8 purity verification. 5 µg of rAra h 8 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.

Reactivity of rAra h 8 with sIgE antibodies in plasma of a patient with confirmed presence (routine clinical test) of specific IgE antibodies to peanut allergen extract.

Western blotting analysis was performed on recombinant allergen rAra h 8 mixed and heated (100°C, 5 min) with reducing SDS loading buffer. Sample was resolved using 15% SDS-PAGE gel. Nitrocellulose membrane blot was stained with Ponceau S to verify protein transfer. After thorough washing and blocking steps, the membrane was incubated with plasma of an allergic patient (1:10). Captured rAra h 8 sIgE antibodies were detected by biotinylated anti-human IgE secondary antibody (2 μg/ml) followed by IRDye 800CW Streptavidin (1:5000).