rAra h 2 (Arachis hypogaea 2.0201)

rAra h 2 (Arachis hypogaea 2.0201)
Regulatory status
RUO
Format
Purified Azide Free
Application
Variant
0.1 mg
10-P208-C100
In stock
242.00 USD
Variant
0.1 mg
10-P208-C100
In stock
242.00 USD
Product details
Description
Images
SDS download
Application
Description
Recombinant protein rAra h 2 is expressed in Escherichia coli. DNA sequence encoding 164 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 19,5 kDa.
Contents
Recombinant protein rAra h 2 - 1 vial (0.1 mg), lyophilized.
Reconstitute with sterile deionized water with or without azide.
Preparation
Purified by sequential steps of ion exchange and affinity chromatography. Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0).
Storage and handling
Store at -20°C to -80°C. Reconstitute in sterile deionized water. Use reconstituted product immediately or aliquot for further storage at -20°C to -80°C.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
UniProt ID Q6PSU2-1
10-P208_čistota
Recombinant allergen rAra h 2 purity verification. 5 µg of rAra h 2 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.
10-P208_WB
Presence of sIgE antibodies specific to recombinant allergens Ara h 1, Ara h 2, and Ara h 8 was analysed in plasma of a patient with confirmed presence (routine clinical test) of specific IgE antibodies to peanut allergen extract.

Western blotting analysis was performed on recombinant allergens rAra h 1, rAra h 2, and rAra h 8 mixed and heated (100°C, 5 min) with reducing SDS loading buffer. Samples were resolved using 15% SDS-PAGE gel. Nitrocellulose membrane blot was incubated with plasma of an allergic patient (1:10). Captured sIgE antibodies were detected by biotinylated anti-human IgE secondary antibody (2 μg/ml) followed by IRDye 800CW Streptavidin (1:5000).

The analysed patient plasma contained sIgE antibodies specific to Ara h 2.
Variant
0.1 mg
10-P208-C100
In stock
242.00 USD
Variant
0.1 mg
10-P208-C100
In stock
242.00 USD

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