Human sHLA-G ELISA

Human sHLA-G ELISA
Regulatory status
RUO
Reactivity
Human
Application
Variant
96 tests
RD194070100R
Delivery 1 week
874.00 USD
Variant
96 tests
RD194070100R
Delivery 1 week
874.00 USD
Product details
Images
References
SDS download
Application
Reactivity
Human
Description
The sHLA-G ELISA kit is a sandwich enzyme immunoassay for the quantitative measurement of soluble forms of human leukocyte antigen G (sHLA-G). The kit measures shedded HLA-G1 and HLA-G5 in EDTA plasma, amniotic fluid, or cell culture supernatant.
Contents
Antibody coated microtiter strips, ready to use, 96 wells
Conjugate solution concentrated (100x), 0.13 ml
Master calibrator, lyophilized, 2 vials
Conjugate diluent, ready to use, 13 ml
Dilution buffer 1, ready to use, 20 ml
Dilution buffer 2, ready to use, 13 ml
Wash solution concentrated (10x), 100 ml
Substrate solution, ready to use, 13 ml
Stop solution, ready to use, 13 ml
Storage and handling
Store at 2-8°C. Do not freeze.
RD194070100R_ELISA
A typical calibration curve for sHLA-G ELISA kit. In the rough 100 U/ml = approximately 40-50 ng/ml.
RD194070100R_ELISA 2
Summary of sHLA-G ELISA kit assay procedure.

Product specific references:

Luque J, Torres MI, Aumente MD, Marin J, Garcia-Jurado G, Gonzalez R, Pascual D, Guerra N, Lopez-Rubio F, Alvarez-Lopez MR, Arizon JM, Pena J: Soluble HLA-G in heart transplantation: their relationship to rejection episodes and immunosuppressive therapy. Hum Immunol. 2006 Apr-May;67(4-5):257-63.
PubMed
Torres MI, Lopez-Casado MA, Luque J, Pena J, Rios A: New advances in coeliac disease: serum and intestinal expression of HLA-G. Int Immunol. 2006 May;18(5):713-8.
PubMed
Desai N, Filipovits J, Goldfarb J: Secretion of soluble HLA-G by day 3 human embryos associated with higher pregnancy and implantation rates: assay of culture media using a new ELISA kit. Reprod Biomed Online. 2006 Aug;13(2):272-7.
PubMed
White SR, Loisel DA, McConville JF, Stern R, Tu Y, Marroquin BA, Noth I, Ober C: Levels of soluble human leukocyte antigen-G are increased in asthmatic airways. Eur Respir J. 2010 Apr;35(4):925-7.
PubMed
Basturk B, Karakayali F, Emiroglu R, Sozer O, Haberal A, Bal D, Haberal M: Human leukocyte antigen-G, a new parameter in the follow-up of liver transplantation. Transplant Proc. 2006 Mar;38(2):571-4.
PubMed
Jiao F, Zhou J, Sun H, Song X, Song Y: Plasma soluble human leukocyte antigen G predicts the long-term prognosis in patients with colorectal cancer. Transl. Cancer Res. 2020;9()6:4011-4019.
PubMed
Li JB, Ruan YY, Hu B, Dong SS, Bi TN, Lin A, Yan WH: Importance of the plasma soluble HLA-G levels for prognostic stratification with traditional prognosticators in colorectal cancer. Oncotarget 2017 Jul 25;8(30):48854-48862.
PubMed
Provatopoulou X, Kalogera E, Sagkriotis A, Zagouri F, Nonni A, Zografos GC, Gounaris A: Soluble human leukocyte antigen-G expression in patients with ductal and lobular breast malignancy. Anticancer Res. 2012 Mar;32(3):1021-6.
PubMed
Keskin F, Karatas A, Albayrak M, Biyik I, Erkan M, Demirin H, Dilbaz S: Maternal serum soluble HLA-G levels in missed abortions. Medicina (Kaunas) 2013;49(10):435-8.
PubMed
Rasmussen M, Dahl M, Buus S, Djurisic S, Ohlsson J, Hviid T: Evaluation of a competitive enzyme-linked immunosorbent assay for measurements of soluble HLA-G protein. Tissue Antigens 2014 Aug;84(2):206-15.
PubMed
Variant
96 tests
RD194070100R
Delivery 1 week
874.00 USD
Variant
96 tests
RD194070100R
Delivery 1 week
874.00 USD