Exbio — Research products — Kits — ApoFlowEx FITC Kit

ApoFlowEx FITC Kit

Regulatory status

RUO

Application

FC (QC tested)

Variant

100 tests

ED7044

In stock

220.00 USD

Variant

100 tests

ED7044

In stock

220.00 USD

Contact distributor

Product details

Images

References

IFU download

SDS download

Application

FC (QC tested)

Description

The ApoFlowEx FITC Kit is intended for differentiation of early apoptotic, necrotic and viable cells.

Contents

ED7044-1 Annexin V-FITC – 1 vial, 0.5 ml of Ready-to-Use solution. No reconstitution is necessary. Reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide. Sufficient for 100 tests.
ED7044-2 Propidium Iodide – 1 vial, 0.5 ml of Ready-to-Use solution in deionized water (0.1 mg/ml). Sufficient for 100 tests.
ED7044-3 Annexin V Binding Buffer (10×) – 1 vial, 5 ml of 10× concentrated filter-sterilized solution (0.1M HEPES/NaOH, pH 7.4, 1.4M NaCl, 25mM CaCl₂). Dilute 10× in deionized water prior to use.

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Flow cytometry staining of apoptotic JURKAT cells with ApoFlowEx FITC Kit (annexin V FITC and PI).

Product specific references:

Drápela S, Khirsariya P, van Weerden WM, Fedr R, Suchánková T, Búzová D, Červený D, Hampl A, Puhr M, Watson WR, Culig Z, Krejčí L, Paruch K, Souček K: The CHK1 inhibitor MU380 significantly increases the sensitivity of human docetaxel‐resistant prostate cancer cells to gemcitabine through the induction of mitotic catastrophe. Molecular Oncology 14, Issue 10.
PubMed

Vahidi R, Safi S, Farsinejad A, Panahi N: Citrate and celecoxib induce apoptosis and decrease necrosis in synergistic manner in canine mammary tumor cells. Cell. Mol. Biol. 2015, 61 (5): 22-28.
PubMed

Pavliukeviciene B, Zentelyte A, Jankunec M, Valiuliene G, Talaikis M, Navakauskiene R, Niaura G, Valincius G: Amyloid β oligomers inhibit growth of human cancer cells. PLoS One. 2019 Sep 11;14(9):e0221563.
PubMed

Rahimifard M, Baeeri M, Bahadar H, Moini-Nodeh S, Khalid M, Haghi-Aminjan H, Mohammadian H, Abdollahi M: Therapeutic Effects of Gallic Acid in Regulating Senescence and Diabetes; an In Vitro Study. Molecules 2020 Dec 11;25(24):5875.
PubMed

Rahmani A, Mousavi HZ, Salehi R, Bagheria A: Novel pH-sensitive and biodegradable micelles for the combined delivery of doxorubicin and conferone to induce apoptosis in MDA-MB-231 breast cancer cell line. RSC Advances 2020, Issue 49
PubMed

Stefanache T, Forna N, Badescu M, Jitaru D, Dragos ML, Rezus C, Diaconescu BM, Badulescu O, Rezus E, Ciocoiu M, Badescu C: Modulation of the activity of certain genes involved in tumor cell metabolism in the presence of the cytotoxic peptides defensin and cathelicidin LL37. Exp Ther Med. 2019 Dec;18(6):5033-5040.
PubMed

Krátká Z, Luxová Š, Vik V: Flow cytometry analysis of sperm quality in sub-fertile men. Ces Urol 2017; 21(3): 277-286
PubMed

Doustvandi MA, Mohammadnejad F, Mansoori B, Tajalli H, Mohammadi A, Mokhtarzadeh A, Baghbani E, Khaze V, Hajiasgharzadeh K, Moghaddam MM, Hamblin MR, Baradaran B: Photodynamic therapy using zinc phthalocyanine with low dose of diode laser combined with doxorubicin is a synergistic combination therapy for human SK-MEL-3 melanoma cells. Photodiagnosis Photodyn Ther. 2019 Dec;28:88-97.
PubMed

Pedram S, Mohammadirad A, Rezvanfar MA, Navaei-Nigjeh M, Baeeri M, Abdollahi M: On the protection by the combination of CeO2 nanoparticles and sodium selenite on human lymphocytes against chlorpyrifos-induced apoptosis in vitro. CELL JOURNAL(Yakhteh), 2015, 17, 2, 361-371.
PubMed

Payandeh Z, Tazehkand AP, Mansoori B, Khaze V, Samadi N, Baradaran B: The potential role of Nrf2-PD-L1 axis in promoting of oxaliplatin resistance in colon cancer cells. doi.org/10.21203/rs.3.rs-58926/v1
PubMed

Lasta CS, Hlavac N, Marcondes NA, Dalmolin ML, Terra SR, de Almeida Lacerda L, Faulhaber GA, González FH: Quality control in veterinary blood banks: evaluation of canine platelet concentrates stored for five days. BMC Veterinary Res. 2020, 16:25
PubMed

Ghaemmaleki F, Mohammadi P, Baeeri M, Navaei-Nigjeh M, Abdollahi M, Mostafalou S: Estrogens counteract tributyltin-induced toxicity in the rat islets of Langerhans. Heliyon 2020, 6, 3, e03562
PubMed

Haghi-Aminjan H, Baeerib M, Rahimifard M, Alizadeh A, Hodjat M, Hassani S, Asghari MH, Abdollahi A, Didari T, Hosseini R, Sharifzadeh M, Abdollahi M: The role of minocycline in alleviating aluminum phosphide-induced cardiac hemodynamic and renal toxicity. Env. Toxicol. Pharmacol. 64 (2018) 26-40.
PubMed

Further IFU language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
ED7044_TDS_v4_EN.pdf