Anti-Orf1 (N. meningitidis) Purified

Anti-Orf1 (N. meningitidis) Purified
Regulatory status
RUO
Antigen
Orf1 (N. meningitidis)
Clone
PAb (489)
Format
Purified
Reactivity
Neisseria meningitidis
Application
Variant
0.1 mg
11-489-C100
In stock
180.00 USD

0.025 mg
11-489-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
References
Isotype
Rabbit polyclonal
Specificity
The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.
Application
Application details
Western blotting: Recommended dilution 0.5-1 μg/ml, reducing conditions.
Reactivity
Neisseria meningitidis
Immunogen
Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.
Concentration
1 mg/ml
Preparation
Purified from rabbit serum by affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
FrpD
Antigen description
Orf1 (FrpD) is a <i>Neisseria meningitidis</i> outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in <i>Escherichia coli </i>revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.

Product specific references:

Prochazkova K, Osicka R, Linhartova I, Halada P, Sulc M, Sebo P: The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC. J Biol Chem. 2005 Feb 4;280(5):3251-8. Epub 2004 Nov 2.
PubMed
Variant
0.1 mg
11-489-C100
In stock
180.00 USD

0.025 mg
11-489-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD