Exbio — Research products — Antibodies — Microbiology and virology — Anti-Orf1 (N. meningitidis) Purified

Anti-Orf1 (N. meningitidis) Purified

Regulatory status

RUO

Antigen

Orf1 (N. meningitidis)

Clone

PAb (489)

Format

Purified

Reactivity

Neisseria meningitidis

Application

Variant

0.1 mg

11-489-C100

In stock

198.00 USD

0.025 mg

11-489-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-489-C100

In stock

198.00 USD

0.025 mg

11-489-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

References

SDS download

Isotype

Rabbit polyclonal

Specificity

The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.

Application

Application details

Western blotting: Recommended dilution 0.5-1 μg/ml, reducing conditions.

Reactivity

Neisseria meningitidis

Immunogen

Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.

Concentration

1 mg/ml

Preparation

Purified by ligand affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

FrpD

Antigen description

Orf1 (FrpD) is a <i>Neisseria meningitidis</i> outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in <i>Escherichia coli </i>revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.

Product specific references:

Prochazkova K, Osicka R, Linhartova I, Halada P, Sulc M, Sebo P: The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC. J Biol Chem. 2005 Feb 4;280(5):3251-8. Epub 2004 Nov 2.
PubMed