Exbio — Research products — Antibodies — Tags and labels — Anti-mPlum purified

Anti-mPlum purified

Regulatory status

RUO

Antigen

mPlum

Clone

PAb (919)

Format

Purified

Application

WB, FC (QC tested), ICC

Variant

0.1 mg

11-919-C100

In stock

198.00 USD

Variant

0.1 mg

11-919-C100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Rabbit polyclonal

Specificity

The rabbit polyclonal antibody PAb (919) recognizes a far red fluorescent protein tag mPlum.

Application

WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular staining or intracellular staining - depending on expression.
Western blotting: Recommended dilution: 1-2 μg/ml.

Immunogen

mPlum from Discosoma sp.

Concentration

1 mg/ml

Preparation

Purified by ligand affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

The mPlum is a red fluorescent protein with excitation maximum 589 nm and emission maximum 650 nm. It has around 29 kDa, and it is being used as a fluorescent tag in expression systems.

UniProt ID Q5S3G7

Western blotting analysis of mPlum fluorescent protein using rabbit polyclonal antibody PAb (919) on lysates of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs (HEK293T/17 cells transfected with YFP/GPI; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rabbit anti-mPlum polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for mPlum protein at approximately 30 kDa.

Flow cytometry surface staining pattern of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs stained using anti-mPlum Purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC).

Separation of HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs stained anti-mPlum Purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mPlum/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mPlum/YFP cell suspension.